Preparation of asparagine-linked monoglucosylated high-mannose-type oligosaccharide from egg yolk

被引：16

作者：

Wang, Ning ^{[1
,2
]}

Seko, Akira ^{[1
]}

Takeda, Yoichi ^{[1
]}

Ito, Yukishige ^{[1
,2
]}

机构：

[1] Japan Sci & Technol Agcy JST, ERATO, Ito Glycotril Project, Wako, Saitama 3510198, Japan

[2] RIKEN, Synthet Cellular Chem Lab, Wako, Saitama 3510198, Japan

来源：

CARBOHYDRATE RESEARCH | 2015年 / 411卷

关键词：

Monoglucosylated high-mannose-type; oligosaccharide; Glycopeptide; Egg yolk; IgY; GLYCOPROTEIN QUALITY-CONTROL; GLYCOSYLATION; THERAPEUTICS; STRATEGY;

D O I：

10.1016/j.carres.2015.04.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Monoglucosylated high-mannose-type glycan (Glc(1)Man(9)GlcNAc(2): G1M9) is well-known as a key glycoform in the glycoprotein folding process, which is specifically recognized by lectin chaperones calnexin (CNX) and calreticulin (CRT) in the endoplasmic reticulum (ER). In this work, we developed an efficient method for the preparation of G1M9-Asn. The G1M9-Asn was obtained from the IgY-rich fraction derived from hen egg yolk by the digestion with pronase. The a-amino group of asparagine in G1M9-Asn was protected with the 9-fluorenylmethyloxycarbonyl (Fmoc) group and the labeled glycans were subsequently purified using high performance liquid chromatography (HPLC). This method will provide useful substrates for analysis of the glycoprotein folding cycle in the ER. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：37 / 41

页数：5

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