Synthesis of highly dispersed nanoscaled CoQ10 liposome by supercritical fluid

The nanoscaled CoQ(10) liposome were synthesized by Rapid Expansion of Supercritical Solutions (RESS). Transmission electron microscopy (TEM) was used to characterize the particle size of CoQ(10) liposome, and the UV spectrum was employed to evaluate the product's trapping efficiency. A series of CoQ(10) liposome samples were synthesized by RESS under different pressures. The trapping efficiency of the CoQ(10) liposome was up to 90% when the enduring pressure was at 200-300 bar, but the trapping efficiency was distinctly decreased when the enduring pressure is lower than 200 bar or higher than 300 bar. The high resolution TEM images exhibited that the particle size of CoQ(10) liposome was only 20-40 nm, probably because CO2 molecules act as the membranes between the CoQ(10) liposome particles in this process, which prevent the agglomeration of liposome with each other. (C) 2014 Elsevier B.V. All rights reserved.