Application of MS-based methods in protein drug development

被引：0
|
作者：
Bobst, Cedric E. [1 ]
Wang, Shunhai [1 ]
Kaltashov, Igor A. [1 ]
机构：
[1] Univ Massachusetts Amherst, Dept Chem, Amherst, MA 01003 USA
来源：
CHIMICA OGGI-CHEMISTRY TODAY | 2012年 / 30卷 / 01期
基金：
美国国家卫生研究院;
关键词：
IONIZATION MASS-SPECTROMETRY; THERAPEUTIC ANTIBODIES; SERUM TRANSFERRIN; ESI-MS;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Mass spectrometry is a proven workhorse in the analytical tool box of the biopharmaceutical industry, although its use seems relegated to the characterization of covalent structure. The utility of mass spectrometry based methods has expanded beyond basic characterization. Methods well suited to probe the conformational properties of protein drugs as well as their interactions with physiological partners in vitro are discussed in the context of drug development. Additionally, the use of quantitative MS-based methods to probe the pharmacokinetic profiles of a protein drug is explored.
引用
收藏
页码：68 / 70
页数：3
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