Pilt, a novel peripheral membrane protein at tight junctions in epithelial cells

被引:33
|
作者
Kawabe, H
Nakanishi, H
Asada, M
Fukuhara, A
Morimoto, K
Takeuchi, M
Takai, Y
机构
[1] Osaka Univ, Grad Sch Med, Fac Med, Dept Mol Biol & Biochem, Suita, Osaka 5650871, Japan
[2] KAN Res Inst Inc, Shimogyo Ku, Kyoto 6008815, Japan
关键词
D O I
10.1074/jbc.M107335200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Tight junctions (TJs) serve as a barrier that prevents solutes and water from passing through the paracellular pathway, and as a fence between the apical and basolateral plasma membranes in epithelial cells. TJs consist of transmembrane proteins (claudin, occludin, and JAM) and many peripheral membrane proteins, including actin filament (F-actin)-binding scaffold proteins (ZO-1, -2, and -3), non-F-actin-binding scaffold proteins (MAGI-1), and cell polarity molecules (ASIP/PAR-3 and PAR-6). We identified here a novel peripheral membrane protein at TJs from a human cDNA library and named it Pilt (for protein incorporated later into TJs), because it was incorporated into TJs later after the claudin-based junctional strands were formed. Pilt consists of 547 amino acids with a calculated M-r of 60,704. Pilt has a proline-rich domain. In cadherin-deficient L cells stably expressing claudin or JAM, Pilt was not recruited to claudin-based or JAM-based cell-cell contact sites, suggesting that Pilt does not directly interact with claudin or JAM. The present results indicate that Pilt is a novel component of TJs.
引用
收藏
页码:48350 / 48355
页数:6
相关论文
共 50 条
  • [1] Identification of a novel protein, LYRIC, localized to tight junctions of polarized epithelial cells
    Britt, DE
    Yang, DF
    Yang, DQ
    Flanagan, D
    Callanan, H
    Lim, YP
    Lin, SH
    Hixson, DC
    [J]. EXPERIMENTAL CELL RESEARCH, 2004, 300 (01) : 134 - 148
  • [2] Peripheral myelin protein 22 is a novel constituent of tight junctions.
    Notterpek, L
    Roux, K
    Fletcher, BS
    Misko, A
    Sealey, A
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2000, 11 : 512A - 512A
  • [3] OCCLUDIN - A NOVEL INTEGRAL MEMBRANE-PROTEIN LOCALIZING AT TIGHT JUNCTIONS
    FURUSE, M
    HIRASE, T
    ITOH, M
    NAGAFUCHI, A
    YONEMURA, S
    TSUKITA, S
    TSUKITA, S
    [J]. JOURNAL OF CELL BIOLOGY, 1993, 123 (06): : 1777 - 1788
  • [4] EPITHELIAL TIGHT JUNCTIONS
    CEREIJIDO, M
    GONZALEZMARISCAL, L
    CONTRERAS, RG
    [J]. AMERICAN REVIEW OF RESPIRATORY DISEASE, 1988, 138 (06): : S17 - S21
  • [5] A Membrane Fusion Protein αSNAP Is a Novel Regulator of Epithelial Apical Junctions
    Naydenov, Nayden G.
    Brown, Bryan
    Harris, Gianni
    Dohn, Michael R.
    Morales, Victor M.
    Baranwal, Somesh
    Reynolds, Albert B.
    Ivanov, Andrei I.
    [J]. PLOS ONE, 2012, 7 (04):
  • [6] ESTABLISHMENT OF TIGHT JUNCTIONS BETWEEN EPITHELIAL-CELLS
    HUDSPETH, AJ
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (07) : 2711 - 2713
  • [7] Connexins Induce and Maintain Tight Junctions in Epithelial Cells
    Takashi Kojima
    Masaki Murata
    Mitsuru Go
    David C. Spray
    Norimasa Sawada
    [J]. Journal of Membrane Biology, 2007, 217 : 13 - 19
  • [8] Role of nectin in organization of tight junctions in epithelial cells
    Fukuhara, A
    Irie, K
    Yamada, A
    Katata, T
    Honda, T
    Shimizu, K
    Nakanishi, H
    Takai, Y
    [J]. GENES TO CELLS, 2002, 7 (10) : 1059 - 1072
  • [9] Connexins induce and maintain tight junctions in epithelial cells
    Kojima, Takashi
    Murata, Masaki
    Go, Mitsuru
    Spray, David C.
    Sawada, Norimasa
    [J]. JOURNAL OF MEMBRANE BIOLOGY, 2007, 217 (1-3): : 13 - 19
  • [10] CFTR and tight junctions in cultured bronchial epithelial cells
    Nilsson, Harriet E.
    Dragomir, Anca
    Lazorova, Lucia
    Johannesson, Marie
    Roomans, Godfried M.
    [J]. EXPERIMENTAL AND MOLECULAR PATHOLOGY, 2010, 88 (01) : 118 - 127