Cloning, Expression and Identification by Immunohistochemistry of Humanized Single-Chain Variable Fragment Antibody against Hepatitis C Virus Core Protein

被引：5
|
作者：
Lun, Yong-Zhi [1 ,2 ]
Cheng, Jun [2 ]
Zhong, Yan-Wei [2 ]
Yu, Zeng-Guo [1 ]
Wang, Qi [2 ]
Wang, Fang
Feng, Jie [1 ]
机构：
[1] Dalian Univ, Liaoning Prov Univ Key Lab Biophys, Coll Med, Dalian 116622, Peoples R China
[2] Beijing Ditan Hosp, Inst Infect Dis, Beijing 100015, Peoples R China
来源：
POLISH JOURNAL OF MICROBIOLOGY | 2011年 / 60卷 / 01期
关键词：
Hepatitis C virus; core protein; phage display; single-chain variable fragment antibody; ENZYME-IMMUNOASSAY; ANTIGEN;
D O I：
10.33073/pjm-2011-002
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Expression of single-chain variable fragment (scFv) antibodies on the surface of bacteriophage is widely used to prepare antibodies with pre-defined specificities. A phage antibody library containing the gene for scFv antibody against Hepatitis C virus core protein was panned with core protein immobilized on microtiter plate wells. After five rounds of panning 60 phage clones specific to core protein were obtained and one selected clone was sequenced. It was found that the specifically detected antigen consists of 774bp and is capable of encoding 257 amino acids in the patients but not in healthy persons.
引用
收藏
页码：13 / 17
页数：5
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