Comparative Genome Sequence Analysis of Actinobacillus pleuropneumoniae Serovar 8 Isolates From Norway, Denmark, and the United Kingdom Indicates Distinct Phylogenetic Lineages and Differences in Distribution of Antimicrobial Resistance Genes

被引：5

作者：

Cohen, Liza Miriam ^{[1
]}

Bosse, Janine T. ^{[2
]}

Stegger, Marc ^{[3
]}

Li, Yanwen ^{[2
]}

Langford, Paul R. ^{[2
]}

Kielland, Camilla ^{[1
]}

Klem, Thea Blystad ^{[4
]}

Gulliksen, Stine Margrethe ^{[5
]}

Ranheim, Birgit ^{[1
]}

Grontvedt, Carl Andreas ^{[4
]}

Angen, Oystein ^{[3
]}

机构：

[1] Norwegian Univ Life Sci NMBU, Fac Vet Med, Dept Prod Anim Clin Sci, Oslo, Norway

[2] Imperial Coll London, Dept Infect Dis, Sect Paediat Infect Dis, London, England

[3] Statens Serum Inst, Dept Bacteria Parasites & Fungi, Copenhagen, Denmark

[4] Norwegian Vet Inst, Oslo, Norway

[5] Animalia, Oslo, Norway

来源：

FRONTIERS IN MICROBIOLOGY | 2021年 / 12卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

Actinobacillus pleuropneumoniae; phylogeny; whole genome sequencing; antimicrobial resistance genes; evolution; serovar; 8; INFECTION PATTERNS; COLONIZATION; TRANSMISSION; DIVERSITY; ALGORITHM; SEROTYPE; ENGLAND; HERDS; PIGS;

D O I：

10.3389/fmicb.2021.729637

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia, a disease of major impact on pig health, welfare, and productivity globally. Serovar 8 (APP) is the predominant clinical serovar in Norway and the United Kingdom (UK), and has been isolated from clinical cases in Denmark. The primary objective of this study was to characterize the genetic variability of isolates of A. pleuropneumoniae APP8 in the Norwegian population. The secondary objectives were to determine the within-host variability of APP8; to compare the APP8 bacterial populations in Norway, Denmark, and the UK, including antimicrobial resistance (AMR) gene profiles and to assess the effect of national differences in antimicrobial drug use and restricted animal movement on the occurrence of resistance. Isolates of APP8 from the UK (n=67), Denmark (n=22), and Norway (n=123) collected between 1983 and 2020 were compared using whole genome sequencing. To investigate genetic variability within individual hosts, an additional 104 APP8 isolates from the lungs of six Norwegian pigs were compared. Very low within-host variation was observed (<= 2 single nucleotide polymorphisms). The phylogeny of 123 Norwegian APP8 isolates from 76 herds revealed some within-herd genetic variation, but substantial geographical clustering. When inferring the relatedness of the three international APP8 collections, the topology highlighted the existence of two distinct monophyletic branches characterized by the Norwegian and UK isolates, respectively. Three Danish isolates were scattered across the UK branch, whereas the remaining 19 Danish isolates clustered in two monophyletic groups nested in the Norwegian branch. Coalescence analysis, performed to estimate the divergences from a common ancestor, indicated a last common ancestor several centuries ago. The phylogenetic analyses also revealed striking differences in occurrence of AMR genes, as these were 23-times more prevalent among the UK isolates than among the Norwegian isolates. An increased understanding of the effects of population strategies is helpful in surveillance and control of infectious diseases.

引用

页数：13