Interaction of Single Cells with 2D Organic Monolayers: A Scanning Electrochemical Microscopy Study

被引：16
|
作者：
Bartolini, Luca [1 ,3 ]
Malferrari, Marco [1 ]
Lugli, Francesca [1 ]
Zerbetto, Francesco [1 ]
Paolucci, Francesco [1 ]
Pelicci, Pier Giuseppe [3 ,4 ]
Albonetti, Cristiano [2 ]
Rapine, Stefania [1 ,3 ]
机构：
[1] Univ Bologna, Dept Chem G Ciamician, Via F Selmi 2, I-40126 Bologna, Italy
[2] CNR, CNR, ISMN, Via P Gobetti 101, I-40129 Bologna, Italy
[3] European Inst Oncol IEO, Dept Expt Oncol, Via Ripamonti 435, I-20141 Milan, Italy
[4] Univ Milan, Dept Oncol & Hematooncol, Via Santa Sofia 9, I-20122 Milan, Italy
来源：
CHEMELECTROCHEM | 2018年 / 5卷 / 20期
关键词：
single cell; SECM; cell morphology; cell contact angle; organic monolayers; LIVING CELLS; ADHESION; DYNAMICS; BEHAVIOR; SURFACE; SHAPE; TIME;
D O I：
10.1002/celc.201800731
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
Cell shape is a valuable indicator of cell health status and interactions with the extracellular materials. With the aim to investigate how individual cells respond to chemical microenvironments, we employed 2D monomolecular layers of small organic molecules, characterized by very low roughness. MCF10A cells were grown on these surfaces and characterized by using scanning electrochemical microscopy. The single cell morphologies were analyzed to define a "figure of merit" for the different cell-surface interactions. Such "figure of merit" quantifies the interaction with the microenvironment. This method is applicable to any cell state (metastatic, physiological or pathological) and any surface.
引用
收藏
页码：2975 / 2981
页数：7
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