A study of metabolic compartmentation in the rat heart and cardiac mitochondria using high-resolution magic angle spinning 1H NMR spectroscopy

被引:40
|
作者
Bollard, ME
Murray, AJ
Clarke, K
Nicholson, JK
Griffin, JL
机构
[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England
[2] Univ Oxford, Physiol Lab, Oxford OX1 3PT, England
[3] Univ London Imperial Coll Sci Technol & Med, Div Biomed Sci, London SW7 2AY, England
关键词
magic angle spinning; mitochondrial respiration; tricarboxylic acid cycle;
D O I
10.1016/S0014-5793(03)00969-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
High-resolution magic angle spinning (MAS) H-1 nuclear magnetic resonance (NMR) spectroscopy is increasingly being used to monitor metabolic abnormalities within cells and intact tissues. Many toxicological insults and metabolic diseases affect subcellular organelles, particularly mitochondria. In this study high-resolution H-1 NMR spectroscopy was used to examine metabolic compartmentation between the cytosol and mitochondria in the rat heart to investigate whether biomarkers of mitochondrial dysfunction could be identified and further define the mitochondrial environment. High-resolution MAS spectra of mitochondria revealed NMR signals from lactate, alanine, taurine, choline, phosphocholine, creatine, glycine and lipids. However, spectra from mitochondrial extracts contained additional well-resolved resonances from valine, methionine, glutamine, acetoacetate, succinate, and aspartate, suggesting that a number of metabolites bound within the mitochondrial membranes occur in 'NMR invisible' environments. This effect was further investigated using diffusion-weighted measurements of water and NMR spectroscopy during state 2 and state 3 respiration. State 3 respiration caused a decrease in the resonance intensity of endogenous succinate compared with state 2 respiration, suggesting that coupled respiration may also modulate the NMR detection of metabolites within mitochondria. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
引用
收藏
页码:73 / 78
页数:6
相关论文
共 50 条
  • [1] High-resolution 1H NMR spectroscopy in organs and tissues using slow magic angle spinning
    Wind, RA
    Hu, JZ
    Rommereim, DN
    [J]. MAGNETIC RESONANCE IN MEDICINE, 2001, 46 (02) : 213 - 218
  • [2] High-resolution 1H NMR spectroscopy in rat liver using magic angle turning at a 1 Hz spinning rate
    Hu, JZ
    Rommereim, DN
    Wind, RA
    [J]. MAGNETIC RESONANCE IN MEDICINE, 2002, 47 (05) : 829 - 836
  • [3] High-resolution 1H and 1H-13C magic angle spinning NMR spectroscopy of rat liver
    Bollard, ME
    Garrod, S
    Holmes, E
    Lincoln, JC
    Humpfer, E
    Spraul, M
    Nicholson, JK
    [J]. MAGNETIC RESONANCE IN MEDICINE, 2000, 44 (02) : 201 - 207
  • [4] Metabolic profiling of listeria rhombencephalitis in small ruminants by 1H high-resolution magic angle spinning NMR spectroscopy
    Precht, Christina
    Diserens, Gaelle
    Vermathen, Martina
    Oevermann, Anna
    Lauper, Josiane
    Vermathen, Peter
    [J]. NMR IN BIOMEDICINE, 2018, 31 (12)
  • [5] Monitoring apoptosis in intact cells by high-resolution magic angle spinning 1H NMR spectroscopy
    Wylot, Marta
    Whittaker, David T. E.
    Wren, Stephen A. C.
    Bothwell, John H.
    Hughes, Leslie
    Griffin, Julian L.
    [J]. NMR IN BIOMEDICINE, 2021, 34 (03)
  • [6] Metabolic profile of intact tissue from uterine leiomyomas using high-resolution magic-angle-spinning 1H NMR spectroscopy
    Mazzei, Pierluigi
    Piccolo, Alessandro
    Nugnes, Loredana
    Mascolo, Massimo
    De Rosa, Gaetano
    Staibano, Stefania
    [J]. NMR IN BIOMEDICINE, 2010, 23 (10) : 1137 - 1145
  • [7] 1H high-resolution magic angle spinning NMR spectroscopy for the investigation of a Ras lipopeptide in a lipid membrane
    Huster, D
    Kuhn, K
    Kadereit, D
    Waldmann, H
    Arnold, K
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2001, 40 (06) : 1056 - +
  • [8] High-resolution 1H NMR spectroscopy in a live mouse subjected to 1.5 Hz magic angle spinning
    Wind, RA
    Hu, JZ
    Rommereim, DN
    [J]. MAGNETIC RESONANCE IN MEDICINE, 2003, 50 (06) : 1113 - 1119
  • [9] 1H High-resolution magic angle spinning NMR spectroscopy for the investigation of a Ras Lipopeptide in a lipid membrane
    Huster, D.
    Kuhn, K.
    Kadereit, D.
    Waldmann, H.
    Arnold, K.
    [J]. Angewandte Chemie (International Edition in English), 2001, 40 (06): : 1056 - 1058
  • [10] High-resolution 1H NMR spectroscopy of metabolically active microorganisms using non-destructive magic angle spinning
    Hu, JZ
    Wind, RA
    McLean, J
    Gorby, YA
    Resch, CT
    Fredrickson, JK
    [J]. SPECTROSCOPY, 2004, 19 (12) : 98 - 103