A newly established real-time PCR for detection of Borrelia miyamotoi in Ixodes ricinus ticks

被引：23
|
作者：
Reiter, Michael [1 ]
Schoetta, Anna-Margarita [1 ]
Mueller, Andreas [1 ]
Stockinger, Hannes [1 ]
Stanek, Gerold [1 ]
机构：
[1] Med Univ Vienna, Inst Hyg & Appl Immunol, Ctr Pathophysiol Infectiol & Immunol, A-1090 Vienna, Austria
来源：
TICKS AND TICK-BORNE DISEASES | 2015年 / 6卷 / 03期
关键词：
Borrelia; Miyamotoi; Ixodes; Tick; PCR; BURGDORFERI SENSU-LATO; LENGTH POLYMORPHISM ANALYSIS; LYME-DISEASE SPIROCHETE; RELAPSING-FEVER; NORTH-AMERICA; SP-NOV; VECTOR; IDENTIFICATION; PERSULCATUS; SEQUENCE;
D O I：
10.1016/j.ttbdis.2015.02.002
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
A total of 350 ticks collected in Austria were analyzed for the presence of DNA sequences of B. miyamotoi. Three ticks gave positive results in a B. miyamotoi-specific nested PCR. Results were confirmed by sequencing the amplified glpQ gene from the positive samples. Moreover we developed a real-time PCR which unambiguously detected B. miyamotoi in all positive samples. Further genotyping of the samples found 100% identity of the 16S-23S intergenic spacer region with Swedish B. miyamotoi sequences. This is the first detection of the relapsing fever spirochete Borrelia miyamotoi in hard ticks in Austria. The results consolidate the picture of a European-wide distribution of B. miyamotoi and again underscore the need for clinical awareness to clarify possible involvement of this species in human disease. (C) 2015 Elsevier GmbH. All rights reserved.
引用
收藏
页码：303 / 308
页数：6
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