A PCR-RFLP method for the simultaneous detection of Babesia bigemina and Theileria annulata infections in cattle

被引：0

作者：

Ravindran, R. ^{[1
]}

Saravanan, B. C. ^{[1
]}

Rao, J. R. ^{[1
]}

Mishra, A. K. ^{[1
]}

Bansal, G. C. ^{[1
]}

Ray, D. ^{[1
]}

机构：

[1] Indian Vet Res Inst, Div Parasitol, Izatnagar 243122, Uttar Pradesh, India

来源：

CURRENT SCIENCE | 2007年 / 93卷 / 12期

关键词：

Babesia bigemina; cattle; PCR-RFLP; simultaneous detection; Theileria annulata;

D O I：

暂无

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mixed infections due to Theileria annulata and Babesia bigemina are responsible for widespread morbidity and mortality in cattle and buffalo of the tropics and subtropics. In the present communication, a method for the differentiation of these two common tick-borne infections is described. A polymerase chain reaction (PCR) for the amplification of partial 18S small subunit ribosomal DNA fragment from the genomes of B. bigemina and T. annulata was standardized. The PCR amplified a monomorphic DNA fragment of 564 by size, which was sequenced and analysed for the restriction enzyme differentiating these two haemoprotozoans in the presence of the host DNA. The enzyme Cfr131 was found to differentiate the two species.

引用

页码：1840 / 1843

页数：4

共 50 条

[1] Development of a duplex PCR assay for simultaneous detection of Babesia bigemina and Theileria annulata infections in cattle
Maharana, Biswa Ranjan
Ganguly, Anita
Bisla, Ranbir Singh
Kumar, Ankit
Potliya, Sandeep
Arora, Devan
[J]. INDIAN JOURNAL OF ANIMAL SCIENCES, 2018, 88 (04): : 10 - 14
[2] β-Tubulin Gene Based PCR-RFLP Method for Specific Detection of Babesia bigemina and Theileria annulata Isolates
Singh, Harkirat
Cheema, P. S.
Mishra, A. K.
Tewari, A. K.
Rao, J. R.
Ravindran, R.
Ray, D.
Bansal, G. C.
[J]. JOURNAL OF APPLIED ANIMAL RESEARCH, 2010, 37 (02) : 233 - 238
[3] A PCR-RFLP method for specific detection of Theileria annulata
Ravindran, R.
Saravanan, B. C.
Rao, J. R.
Mishra, A. K.
Bansal, G. C.
Ray, D.
[J]. JOURNAL OF APPLIED ANIMAL RESEARCH, 2007, 32 (01) : 111 - 112
[4] Babesia bigemina and Theileria annulata infections in cattle: molecular detection, phylogenetic analysis, and assessment of risk factors
Khan, Majid
Khan, Mehran
Khan, Momin
Batool, Sidra
Tanaka, Tetsuya
Aloufi, Abdulaziz
Almutairi, Mashal M.
Ali, Abid
[J]. TROPICAL ANIMAL HEALTH AND PRODUCTION, 2024, 56 (08)
[5] Prevalence of Theileria annulata and Babesia bigemina in Cattle in the Vicinity of Sanliurfa
Sevgili, Murat
Cakmak, Ayse
Gokcen, Ahmet
Altas, Mehtap Gul
Ergun, Gul
[J]. JOURNAL OF ANIMAL AND VETERINARY ADVANCES, 2010, 9 (02): : 292 - 296
[6] Pentaplex PCR assay for rapid differential detection of Babesia bigemina, Theileria annulata, Anaplasma marginale and Trypanosoma evansi in cattle
Ganguly, Anita
Maharana, Biswa Ranjan
Ganguly, Indrajit
[J]. BIOLOGICALS, 2020, 63 : 81 - 88
[7] Molecular detection and characterization of Theileria annulata, Babesia bovis, and Babesia bigemina infecting cattle and buffalo in southern Egypt
Mahmoud, Hassan Y. A. H.
Rady, Abdelrahman A.
Tanaka, Tetsuya
[J]. PARASITE EPIDEMIOLOGY AND CONTROL, 2024, 25
[8] Development of a multiplex PCR assay for simultaneous detection of Theileria annulata, Babesia bovis and Anaplasma marginale in cattle
Bilgic, Huseyin B.
Karagenc, Tulin
Simuunza, Martin
Shiels, Brian
Tait, Andy
Eren, Hasan
Weir, William
[J]. EXPERIMENTAL PARASITOLOGY, 2013, 133 (02) : 222 - 229
[9] Sero-epidemiology of Theileria annulata and Babesia bigemina infections in Morocco.
El Haj, N
Kachani, M
Bouslikhane, M
Ouhelli, H
Ahami, AT
Katende, J
Morzaria, SP
[J]. REVUE DE MEDECINE VETERINAIRE, 2002, 153 (03) : 189 - 196
[10] A PCR method targeting internal transcribed spacers: the simultaneous detection of Babesia bigemina and Babesia bovis in cattle
Junlong Liu
Guiquan Guan
Aihong Liu
Youquan Li
Hong Yin
Jianxun Luo
[J]. Acta Parasitologica, 2014, 59 : 132 - 138

← 1 2 3 4 5 →