Effect of the long non-coding RNA AC007392.4 on growth, invasion and migration for tongue squamous cell carcinoma

Objectives: Long non-coding RNAs (LncRNA) have been reported to affect the development of many types of tumors. However, the research for relationship between LncRNA AC007392.4 and tongue squamous cell carcinoma cells (TSCC) has not been revealed. In this study, we aim to explore the contribution of LncRNA AC007392.4 to growth, invasion and migration in TSCC cells. Methods: To detect LncRNA AC007392.4 expression with QRT-PCR in 20 patients in TSCC tissue and adjacent normal tissue. Then construct pcDNA3.1 (+) expression vector of LncRNA AC007392.4 and transfected into the Cal-27 cell line. By using MTT, transwell migration/invasion assay and apoptosis analysis, we tested the role of LncRNA AC007392.4 on tongue cancer cell growth, migration and invasion. Results: The results indicated that LncRNA AC007392.4 was lower expression in TSCCs tissue than adjacent normal tissue via QRT-PCR (P<0.05). And overexpression of LncRNA AC007392.4 in Cal-27 cells can promote cell growth (P<0.05) and decrease the cell apoptosis rate (P<0.05), but no effect on cell migration and invasion (P>0.05). Conclusion: We suggest that LncRNA AC007392.4 can influence on TSCC cells growth, but not on invasion and migration, may be an important and useful therapeutic biomarker for TSCC patients in future but need more study.