Comparison of Hepatoprotective effect of Silymarin and Zinc Sulfate against Hepatotoxicity induced by Isoniazid and Rifampicin in albino rats

Liver the most vital organ performing functions like protein synthesis, metabolic processes, clearance of xenobiotic (drug) and various toxins. Anti-tuberculosis drugs induced liver injury is a main cause of acute liver failure (ALF). Current treatments available for liver protection are not ineffective enough. Development of regenerative medicines such as (Zinc sulfate or Silymarine) are in great demand. Supplemental use is effective for prevention of liver damage. Both have antioxidant properties. Hepatic protection of Zinc Sulfate and Silymarin was evaluated and compared against 1st line anti TB (Isoniazid 50mg/kg/day and Rifampicin 100 mg/kg/day). 28 rats were divided into 4 groups G1, G2, G3 and G4. Liver injury was induced by Isoniazid and Rifampicin in G2, G3 and G4. Hepatoprotection of Silymarin (200mg /kg/day) and Zinc sulfate (7mg /kg/day) was evaluated in G3 and G4 respectively by serum ALT, AL P, AST, total bilirubin, total protein, albumin, globulin, A/G ratio (LFTs). There was significant elevation of LFTs in G2, showing induction of hepatotoxicity. The levels of ALT, ALK PO4, AST and Total bilirubin were significantly reduced at day 13 p-value ***< 0.0001 in G3, G4. While values of Total Protein, Albumin, Globulin and A/G ratio significant at day 6. Comparing G3, G4 values of ALT and ALT shows significant difference while ALP, Total Bilirubin, Total Protein, Albumin, Globulin and A/G ratio shows no significant difference.