A protocol for full-rotation soft X-ray tomography of cells

被引：8
|
作者：
Chen, Jian-Hua [1 ,2 ]
Vanslembrouck, Bieke [1 ,2 ]
Ekman, Axel [1 ,2 ]
Cortese, Mirko [3 ]
Bartenschlager, Ralf [3 ,4 ,5 ]
Mcdermott, Gerry [1 ,2 ]
Larabell, Carolyn A. [1 ,2 ]
Le Gros, Mark A. [1 ,2 ]
Weinhardt, Venera [1 ,6 ]
机构：
[1] Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA
[2] Univ Calif San Francisco, Dept Anat, San Francisco, CA 94143 USA
[3] Mol Virol Heidelberg Univ, Dept Infect Dis, D-69120 Heidelberg, Germany
[4] German Ctr Infect Res, Heidelberg Partner Site, D-69120 Heidelberg, Germany
[5] German Canc Res Ctr, Div Virus Associated Carcinogenesis, D-69120 Heidelberg, Germany
[6] Heidelberg Univ, Ctr Organismal Studies, D-69120 Heidelberg, Germany
来源：
STAR PROTOCOLS | 2022年 / 3卷 / 01期
基金：
欧洲研究理事会;
关键词：
IMAGE; VISUALIZATION;
D O I：
10.1016/j.xpro.2022.101176
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The protocol describes step-by-step sample preparation, data acquisition, and segmentation of cellular organelles with soft X-ray tomography. It is designed for microscopes built to perform full-rotation data acquisition on specimens in cylindrical sample holders, such as the XM-2 microscope at the Advanced Light Source, LBNL; however, it might be generalized for similar sample holder designs for both synchrotron and table-top microscopes. For complete details on the use and execution of this profile, please refer to Loconte et al. (2021).
引用
收藏
页数：21
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