Rangelia vitalii, Babesia spp. and Ehrlichia spp. in dogs in Passo Fundo, state of Rio Grande do Sul, Brazil

被引:15
|
作者
Gottlieb, Juliana [1 ]
Andre, Marcos Rogerio [2 ]
Soares, Joao Fabio [3 ]
Goncalves, Luiz Ricardo [2 ]
de Oliveira, Mateus Tonial [1 ]
Costa, Marcio Machado [4 ]
Labruna, Marcelo Bahia [5 ]
Bortolini, Carlos Eduardo [4 ]
Machado, Rosangela Zacarias [2 ]
Botelho Vieira, Maria Isabel [1 ]
机构
[1] Univ Passo Fundo, Programa Posgrad Expt Anim, Passo Fundo, RS, Brazil
[2] Univ Estadual Paulista UNESP, Programa Posgrad Parasitol, Jaboticabal, SP, Brazil
[3] Univ Med Vet Unicruz, Cruz Alta, RS, Brazil
[4] Univ Passo Fundo, Curso Med Vet, Passo Fundo, RS, Brazil
[5] Univ Sao Paulo, Programa Posgrad Epidemiol Expt Aplicada Zoonoses, Dept Med Vet Prevent & Saude Anim, FMVZ, BR-09500900 Sao Paulo, SP, Brazil
来源
关键词
Hemoparasites; piroplasms; sequencing; dogs; nambiuvu; indirect immunofluorescence; MOLECULAR CHARACTERIZATION; CANINE RANGELIOSIS; BORNE DISEASES; 1ST REPORT; INFECTION; PIROPLASM; ALIGNMENT;
D O I
10.1590/S1984-29612016041
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Pathogens transmitted by ticks are an emerging problem worldwide, this study aimed to diagnose the causal agents of infection in dogs presenting suspected hemoparasitoses. Fifty-eight dogs with clinical signs such as depression, hemorrhagic diathesis and fever were evaluated regarding clinical presentation, hemogram, blood smears and serological tests, using the indirect immunofluorescence method for the agents Babesia vogeli and Ehrlichia canis and conventional PCR for Babesia spp. (gene 18S rRNA), Rangelia vitalii (gene 18S rRNA) and Ehrlichia spp. (gene dsb). Five (8.6%) of the 58 dogs were serologically positive for Babesia spp. and three (5.1%) for E. canis. Four dogs (6.8%) were positive for R. vitalii through the molecular diagnosis. The PCR products were sequenced and the DNA from R. vitalii was found to be 99% genetically identical to samples of R. vitalii that had been isolated in Brazil. No presence of Babesia spp. or E. canis was observed through PCR on the dogs evaluated here. The results indicate the presence of R. vitalii and exposure to Babesia spp. and Ehrlichia spp. among the dogs analyzed.
引用
收藏
页码:172 / 178
页数:7
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