Isoenzyme N-Acyl-L-Amino Acid Amidohydrolase NA Increases Ochratoxin A Degradation Efficacy of Stenotrophomonas sp. CW117 by Enhancing Amidohydrolase ADH3 Stability

被引：1

作者：

Chen, Nan ^{[1
]}

Fei, Qingru ^{[1
]}

Luo, Han ^{[1
]}

Fang, Zemin ^{[2
]}

Xiao, Yazhong ^{[2
]}

Du, Zhengjun ^{[1
]}

Zhou, Yu ^{[1
,2
]}

机构：

[1] Anhui Agr Univ, Sch Tea & Food Sci Technol, State Key Lab Tea Plant Biol & Utilizat, Hefei, Peoples R China

[2] Anhui Univ, Sch Life Sci, Hefei, Peoples R China

来源：

MICROBIOLOGY SPECTRUM | 2022年 / 10卷 / 04期

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

food safety; mycotoxin pollution; codegradation; detoxification; isoenzyme; ochratoxin A; ESCHERICHIA-COLI; CARBOXYPEPTIDASE; GENE; DETOXIFICATION; EXPRESSION; VECTORS;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Ochratoxin A (OTA) is a potent mycotoxin mainly produced by toxicogenic strains of Aspergillus spp. and seriously contaminates foods and feedstuffs. OTA detoxification strategies are significant to food safety. A superefficient enzyme ADH3 to OTA hydrolysis was isolated from the difunctional strain Stenotrophomonas sp. CW117 in our previous study. Here, we identified a gene N-acyl-L-amino acid amidohydrolase NA, which is an isoenzyme of ADH3. However, it is not as efficient a hydrolase as ADH3. The kinetic constant showed that the catalytic efficiency of ADH3 (K-cat/K-m = 30,3938 s(-1) center dot mM(-1)) against OTA was 29,113 times higher than that of NA (Kcat/Km = 10.4 s(-1) center dot mM(-1)), indicating that ADH3 was the overwhelming superior detoxifying gene in CW117. Intriguingly, when gene na was knocked out from the CW117 genome, degradation activity of the Delta na mutant was significantly reduced at the first 6 h, suggesting that the two enzymes might have an interactive effect on OTA transformation. Gene expressions and Western blotting assay showed that the Delta na mutant and wildtype CW117 showed similar adh3 expression levels, but na deficiency decreased ADH3 protein level in CW117. Collectively, isoenzyme NA was identified as a factor that improved the stability of ADH3 in CW117 but not as a dominant hydrolase for OTA transformation. IMPORTANCE Ochratoxin A (OTA) is a potent mycotoxin mainly produced by toxicogenic strains of Aspergillus spp. and seriously contaminates foods and feedstuffs. Previous OTA detoxification studies mainly focused on characterizations of degradation strains and detoxifying enzymes. Here, we identified a gene N-acyl-L-amino acid amidohydrolase NA from strain CW117, which is an isoenzyme of the efficient detoxifying enzyme ADH3. Isoenzyme NA was identified as a factor that improved the stability of ADH3 in CW117 and, thus, enhanced the degradation activity of the strain. This is the first study on an isoenzyme improving the stability of another efficient detoxifying enzyme in vivo.

引用

页数：14