A real-time PCR assay for detection of isoniazid resistance in Mycobacterium tuberculosis clinical isolates

被引：5

作者：

Rindi, L

Bianchi, L

Tortoli, E

Lari, N

Bonanni, D

Garzelli, C

机构：

[1] Univ Pisa, Dipartimento Patol Sperimentale Biotecnol Med,Inf, I-56127 Pisa, Italy

[2] Osped Careggi, Lab Microbiol & Virol, Ctr Reg Riferimento Micobatteri, I-50134 Florence, Italy

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2003年 / 55卷 / 03期

关键词：

real-time PCR assay; isoniazid resistance; Mycobacterium tuberculosis;

D O I：

10.1016/S0167-7012(03)00210-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A real-time PCR genotypic assay was developed for the detection of isoniazid (INH) resistance in Mycobacterium tuberculosis. The assay detects mutations C(-15)T and, possibly, G( -24)T in the regulatory region of the inhA gene and proved as sensitive and specific as nucleotide sequencing in all the clinical isolates tested. Our assays mapped the mutations efficiently in 10 out of 35 resistant isolates, thereby covering 29% of all resistant strains. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：797 / 800

页数：4

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