Disposable biosensor based on novel ternary Ru-PEI@PCN-333(Al) self-enhanced electrochemiluminescence system for on-site determination of caspase-3 activity

被引：0

作者：

Luo, Weiwei ^{[1
,3
]}

Chu, Hongyu ^{[2
]}

Wu, Xinzhao ^{[1
]}

Ma, Pinyi ^{[1
]}

Wu, Qiong ^{[2
]}

Song, Daqian ^{[1
]}

机构：

[1] Jilin Univ, Coll Chem, Jilin Prov Res Ctr Engn & Technol Spectral Analyt, Qianjin St 2699, Changchun 130012, Peoples R China

[2] Jilin Univ, Dept Gastrointestinal & Colorectal Surg, China Japan Union Hosp, Sendai St 126, Changchun 130033, Peoples R China

[3] Anshan Normal Univ, Sch Chem & Life Sci, Pingan St 43, Anshan 114005, Peoples R China

来源：

TALANTA | 2022年 / 239卷

基金：

中国国家自然科学基金;

关键词：

Caspase-3; Apoptosis; Electrochemiluminescence (ECL); Metal organic frameworks (MOFs); Screen-printed electrodes (SPEs); Electrochemical sensors; Biosensor; METAL-ORGANIC FRAMEWORK; ELECTROCHEMICAL APPROACH; GOLD NANOPARTICLE; CELL APOPTOSIS; POINT; IMMOBILIZATION; GENERATION; PATHWAYS;

D O I：

10.1016/j.talanta.2021.123083

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The number of death due to cancer-related diseases each year is at the alarming level and is constantly growing. Tools that can effectively and conveniently detect cancer cell apoptosis can play a significant role in cancer research, cancer therapy, and other related industries. Herein, we fabricated, for the first time, an ultrasensitive, disposable, self-enhanced off-on electrochemiluminescence (ECL) biosensor based on ternary Ru-PEI@PCN-333 (Al) system to determine caspase-3 activity, the biomarker of apoptosis. The biosensor had a low detection limit of 0.017 pg/mL and was able to enhance the ECL emission and stability. A solid-state (SS) ECL strategy was adopted to overcome the relatively weak ECL emission due to the long distance between electro-chemiluminophore and electrode surface. The analysis requires only one incubation step, which can significantly reduce the operational complexity and time. The biosensor had higher sensitivity, and the off-on ECL mode was achieved using caspase-3 as a switch. The on-site and rapid detection capability of the biosensor was achieved by the introduction of disposable screen-printed electrodes (SPEs). This study lays a foundation for the development of more advanced, ingenious, portable and reliable ECL devices for biosensing not only caspase-3, but also other bioanalytes.

引用

页数：8