Characterization of Enterobacteria using MALDI-TOF mass spectrometry

被引：30
|
作者：
Pribil, P [1 ]
Fenselau, C [1 ]
机构：
[1] Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA
来源：
ANALYTICAL CHEMISTRY | 2005年 / 77卷 / 18期
关键词：
D O I：
10.1021/ac050737c
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method is proposed for the rapid classification of Gramnegative Enterobacteria using on-slide solubilization and trypsin digestion of proteins, followed by MALDI-TOF MS analysis. Peptides were identified from tryptic digests using microsequencing by tandem mass spectrometry and database searches. Proteins from the outer membrane family (OMP) were consistently identified in the Enterobacteria Escherichia coli, Enterobacter cloacae, Erwinia herbicola, and Salmonella typhimurium. Database searches indicate that these OMP peptides observed are unique to the Enterobacteria order.
引用
收藏
页码：6092 / 6095
页数：4
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