Pyrosequencing-based approach for rapid detection of rifampin-resistant Mycobacterium tuberculosis

被引:23
|
作者
Zhao, JR [1 ]
Bai, YJ
Zhang, QH
Wang, Y
Luo, M
Yan, XJ
机构
[1] Fourth Mil Med Univ, Inst Genet Diag, Xian 710032, Peoples R China
[2] Xian Tuberculosis Hosp, Clin Lab, Xian 710061, Peoples R China
关键词
Mycobacterium tuberculosis; rifampin resistance; polymerase chain reaction; pyrosequencing;
D O I
10.1016/j.diagmicrobio.2004.10.001
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Rifampin resistance in Mycobacterium tuberculosis could be determined within 2 h by using pyrosequencing-based approach. Pyrosequencing results of rifampin-resistant (n = 21) and rifampin-susceptible (n = 20) M. tuberculosis isolates were concordant with those based on drug susceptibility testing and conventional DNA sequencing. Results showed pyrosequencing-based approach is a rapid, sensitive, and efficient detection method of rifampin-resistant M. tuberculosis. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码:135 / 137
页数:3
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