PCR-based selection for the root-knot nematode resistance in tomato

We established a PCR-based method for identifying the root-knot nematode resistant trait in tomato, We synthesized primers encompassing the first intron of acid phosphatase-1(1) gene (Aps-1(1)), which is tightly linked to the resistant trait, and performed a PCR analysis with genomic DNAs of both resistant (Aps-1(1), wild type allele) and susceptible cultivars (Aps-1(+), cultivar allele), A clear polymorphism was detected by digesting the products with several restriction enzymes, Comparing sequences of the first intron region, we found several point mutations and a 12bp-long deletion (insertion), By the PCR analysis using primers encompassing the deletion region, we could detect a clear polymorphism without restriction digestions.