Kinetic properties of DNA migration under clamped homogeneous electric field conditions - DNA size, migration velocities and reorientation time determined in a single clamped homogeneous electric field run

By analyzing DNA migrations per pulse at different pulse times we determined the reorientation times and migration velocities, during and after DNA reorientation of molecules separated under different clamped homogeneous electric field (CHEF) electrophoretic conditions. We obtained functions relating these parameters and DNA migration per pulse with running variables and DNA size. A statistical procedure that validates the predictions of these functions was also proposed using identical data for function fitness and validation. A method is proposed to estimate DNA size, reorientation time and migration velocities using the distance migrated by a DNA molecule in a single CHEF experiment. (C) 1998 Elsevier Science B.V.