Differential sorting of nerve growth factor and brain-derived neurotrophic factor in hippocampal neurons

被引:0
|
作者
Mowla, SJ
Pareek, S
Farhadi, HF
Petrecca, K
Fawcett, JP
Seidah, NG
Morris, SJ
Sossin, WS
Murphy, RA
机构
[1] McGill Univ, Montreal Neurol Inst, Ctr Neuronal Survival, Montreal, PQ H3A 2B4, Canada
[2] McGill Univ, Dept Physiol, Montreal, PQ H3A 2B4, Canada
[3] McGill Univ, Montreal Neurol Inst, Dept Neurol & Neurosurg, Cell Biol Excitable Tissues Grp, Montreal, PQ H3A 2B4, Canada
[4] Clin Res Inst Montreal, Biochem Neuroendocrinol Lab, Montreal, PQ H2W 1R7, Canada
来源
JOURNAL OF NEUROSCIENCE | 1999年 / 19卷 / 06期
关键词
NGF; BDNF; precursor; furin; constitutive secretion; regulated secretion; neurotrophin;
D O I
暂无
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Nerve growth factor (NGF) is released through the constitutive secretory pathway from cells in peripheral tissues and nerves where it can act as a target-derived survival factor. In contrast, brain-derived neurotrophic factor (BDNF) appears to be processed in the regulated secretory pathway of brain neurons and secreted in an activity-dependent manner to play a role in synaptic plasticity. To determine whether sorting differences are intrinsic to the neurotrophins or reflect differences between cell types, we compared NGF and BDNF processing in cultured hippocampal neurons using a Vaccinia virus expression system. Three independent criteria (retention or release from cells after pulse-chase labeling, depolarization-dependent release, and immunocytochemical localization) suggest that the bulk of newly synthesized NGF is sorted into the constitutive, pathway, whereas BDNF is primarily sorted into the regulated secretory pathway. Similar results occurred with AtT 20 cells, including those transfected with cDNAs encoding neurotrophin precursor-green fluorescent protein fusions. The NGF precursor, but not the BDNF precursor, is efficiently cleaved by the endoprotease furin in the trans-Golgi network (TGN). Blocking furin activity in AtT 20 cells with alpha 1-PDX as well as increasing the expression of NGF precursor partially directed NGF into the regulated secretory pathway. Therefore, neurotrophins can be sorted into either the constitutive or regulated secretory pathways, and sorting may be regulated by the efficiency of furin cleavage in the TGN. This mechanism may explain how neuron-generated neurotrophins can act both as survival factors and as neuropeptides.
引用
收藏
页码:2069 / 2080
页数:12
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