Kit ligand promotes the transition from primordial to primary follicles after in vitro culture of ovine ovarian tissue

被引:14
|
作者
Cavalcante, A. Y. P. [1 ]
Gouveia, B. B. [1 ]
Barberino, R. S. [1 ]
Lins, T. L. B. G. [1 ]
Santos, L. P. [1 ]
Goncalves, R. J. S. [1 ]
Celestino, J. J. H. [2 ]
Matos, M. H. T. [1 ]
机构
[1] Fed Univ San Francisco Valley, Nucleus Biotechnol Appl Ovarian Follicle Dev, Petrolina, PE, Brazil
[2] Univ Int Integrat Lusophone African Brazilian, Inst Hlth Sci, Acarape, CE, Brazil
关键词
Activation; Kit ligand; Oocyte; Ovary; Sheep; STEM-CELL FACTOR; GOAT PREANTRAL FOLLICLES; DIFFERENTIATION FACTOR-9; GROWTH; ACTIVATION; OOCYTES; SURVIVAL; HORMONE; MOUSE;
D O I
10.1017/S0967199415000556
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
This study evaluated the effects of kit ligand (KL) on the morphology and development of ovine preantral follicles (fresh control) and after 7 days of in vitro culture in alpha-Minimal Essential Medium (alpha-MEM; control medium) or the presence of KL (1, 10, 50, 100 or 200 ng/ml). There was an increase in the percentage of primary follicles at the concentration of 100 ng/ml KL, compared with the fresh control, control medium (alpha-MEM) and the other KL concentrations. Follicle diameter was significantly higher than the control medium only at concentrations of 50 and 100 ng/ml KL. In conclusion, 100 ng/ml KL promoted the transition from primordial to primary follicles (follicular activation) after in vitro culture of ovine ovarian tissue.
引用
收藏
页码:578 / 582
页数:5
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