A CdtA-CdtC complex can block killing of HeLa cells by Haemophilus ducreyi cytolethal distending toxin

被引：31
|
作者：
Deng, KP [1 ]
Hansen, EJ [1 ]
机构：
[1] Univ Texas, SW Med Ctr, Dept Microbiol, Dallas, TX 75390 USA
来源：
INFECTION AND IMMUNITY | 2003年 / 71卷 / 11期
关键词：
D O I：
10.1128/IAI.71.11.6633-6640.2003
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The cytolethal distending toxin (CDT) of Haemophilus ducreyi is comprised of the CdtA, CdtB, and CdtC proteins, with the CdtB protein having demonstrated enzymatic (i.e., DNase) activity. Using a single recombinant Escherichia coli strain with two plasmids individually containing the H. ducreyi cdtA and cdtC genes, we purified a noncovalent CdtA-CdtC complex. Incubation of this CdtA-CdtC complex with HeLa cells blocked killing of these cells by CDT holotoxin. Furthermore, the addition of purified recombinant CdtB to HeLa cells preincubated with the CdtA-CdtC complex resulted in the killing of these human epithelial cells.
引用
收藏
页码：6633 / 6640
页数：8
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  • [1] Interactions of Campylobacter jejuni cytolethal distending toxin subunits CdtA and CdtC with HeLa cells
    Lee, RB
    Hassane, DC
    Cottle, DL
    Pickett, CL
    [J]. INFECTION AND IMMUNITY, 2003, 71 (09) : 4883 - 4890
  • [2] Endocytosis of the CdtA subunit from the Haemophilus ducreyi cytolethal distending toxin
    Robb Huhn, G., III
    Torres-Mangual, Naly
    Clore, John
    Cilenti, Lucia
    Frisan, Teresa
    Teter, Ken
    [J]. CELLULAR MICROBIOLOGY, 2021, 23 (11)
  • [3] The fate of the CdtA subunit from the Haemophilus ducreyi cytolethal distending toxin
    Huhn, Robb
    Torres, Nalysha
    Talyor, Mike
    Teter, Ken
    [J]. BIOPHYSICAL JOURNAL, 2023, 122 (03) : 334A - 334A
  • [4] CdtA, CdtB, and CdtC form a tripartite complex that is required for cytolethal distending toxin activity
    Lara-Tejero, M
    Galán, JE
    [J]. INFECTION AND IMMUNITY, 2001, 69 (07) : 4358 - 4365
  • [5] Cellular internalization of cytolethal distending toxin from Haemophilus ducreyi
    Cortes-Bratti, X
    Chaves-Olarte, E
    Lagergård, T
    Thelestam, M
    [J]. INFECTION AND IMMUNITY, 2000, 68 (12) : 6903 - 6911
  • [6] The impact of Haemophilus ducreyi cytolethal distending toxin on cells involved in immune response
    Svensson, LA
    Tarkowski, A
    Thelestam, M
    Lagergård, T
    [J]. MICROBIAL PATHOGENESIS, 2001, 30 (03) : 157 - 166
  • [7] Cytotoxic activity on HeLa cells of an adenovirus 5 vector expressing cdtB from the Haemophilus ducreyi cytolethal distending toxin
    Wising, Catharina
    Lindholm, Leif
    Lagergard, Teresa
    [J]. HUMAN GENE THERAPY, 2007, 18 (10) : 1001 - 1001
  • [8] Investigation of the interaction among the components of the cytolethal distending toxin of Haemophilus ducreyi
    Deng, KP
    Latimer, JL
    Lewis, DA
    Hansen, EJ
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 285 (03) : 609 - 615
  • [9] The cytolethal distending toxin of Haemophilus ducreyi inhibits endothelial cell proliferation
    Svensson, LA
    Henning, P
    Lagergård, T
    [J]. INFECTION AND IMMUNITY, 2002, 70 (05) : 2665 - 2669
  • [10] Cytolethal distending toxin of Haemophilus ducreyi induces apoptotic death of Jurkat T cells
    Gelfanova, V
    Hansen, EJ
    Spinola, SM
    [J]. INFECTION AND IMMUNITY, 1999, 67 (12) : 6394 - 6402
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