Development of a cell-free system reveals an oxygen-labile step in the maturation of [NiFe]-hydrogenase 2 of Escherichia coli

被引：16

作者：

Soboh, Basem ^{[1
]}

Krueger, Sara ^{[1
]}

Kuhns, Martin ^{[1
]}

Pinske, Constanze ^{[1
]}

Lehmann, Anne ^{[1
]}

Sawers, R. Gary ^{[1
]}

机构：

[1] Univ Halle Wittenberg, Inst Mikrobiol, D-06120 Halle, Saale, Germany

来源：

FEBS LETTERS | 2010年 / 584卷 / 18期

关键词：

NiFe]-hydrogenase; In vitro processing; Hydrogen oxidation; Hyp maturation proteins; HYDROGENASE ISOENZYMES; ACTIVE-SITE; CRYSTAL-STRUCTURES; PROTEINS HYPC; NICKEL; CN; ACTIVATION; EXPRESSION; COMPLEX; ENZYME;

D O I：

10.1016/j.febslet.2010.08.037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By combining extracts from strains lacking genes encoding either the maturation enzymes or the large subunits of hydrogenases 1, 2 and 3 we could reconstitute in vitro under strictly anaerobic conditions 10-15% of the hydrogenase activity present in wild type Escherichia coli extracts. Purified, unprocessed Strep-tagged variants of the hydrogenase 2 large subunit, HybC, isolated from either a Delta hybD (encoding the hydrogenase 2-specific protease) mutant or a strain deficient in HypF could also be matured to active, processed enzyme using this system. These studies reveal that minimally one step early on the hydrogenase maturation pathway is oxygen-labile. (c) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4109 / 4114

页数：6

共 50 条

[1] ON RIBONUCLEIC ACID SYNTHESIZED IN A CELL-FREE SYSTEM OF ESCHERICHIA COLI
OTAKA, E
MITSUI, H
OSAWA, S
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1962, 48 (03) : 425 - &
[2] THE HYDROGENASE OF E-COLI IN THE CELL-FREE STATE .2. THE EFFECT OF CERTAIN INHIBITORS ON HYDROGENASE
JOKLIK, WK
AUSTRALIAN JOURNAL OF EXPERIMENTAL BIOLOGY AND MEDICAL SCIENCE, 1950, 28 (03): : 331 - 338
[3] PHOSPHORYLATION OF ESCHERICHIA-COLI RIBOSOMES .2. REACTION IN CELL-FREE SYSTEM
KUREK, E
GRANKOWSKI, N
GASIOR, E
ACTA MICROBIOLOGICA POLONICA SERIES A-MICROBIOLOGIA GENERALIS, 1972, 4 (04): : 177 - 182
[4] Cloning and expression of the HIV protein in Escherichia coli cell-free system
Chen, Haiqin
Xu, Zhinan
Yin, Xiufei
Cen, Peilin
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2007, 77 (02) : 347 - 354
[5] TRANSLATION OF POLIOVIRUS RNA BY AN ESCHERICHIA-COLI CELL-FREE SYSTEM
REKOSH, D
LODISH, HF
BALTIMORE, D
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1969, 34 : 747 - +
[6] RNA SYNTHESIS IN CRUDE CELL-FREE SYSTEM OF ESCHERICHIA-COLI
KASTRIKI.LN
VOPROSY MEDITSINSKOI KHIMII, 1972, 18 (05): : 521 - &
[7] Cloning and expression of the HIV protein in Escherichia coli cell-free system
Haiqin Chen
Zhinan Xu
Xiufei Yin
Peilin Cen
Applied Microbiology and Biotechnology, 2007, 77 : 347 - 354
[8] FUNCTION OF RETICULOCYTE RIBONUCLEIC ACID IN ESCHERICHIA COLI CELL-FREE SYSTEM
DRACH, JC
LINGREL, JB
BIOCHIMICA ET BIOPHYSICA ACTA, 1966, 129 (01) : 178 - &
[9] DEGRADATION OF 5' ADENOSINE MONOPHOSPHATE IN A CELL-FREE SYSTEM OF ESCHERICHIA COLI
BRANA, H
FOLIA MICROBIOLOGICA, 1967, 12 (01) : 1 - +
[10] DISCONTINUOUS REPLICATION OF DNA IN A CELL-FREE SYSTEM FROM ESCHERICHIA-COLI
MAJUMDAR, C
FEDERATION PROCEEDINGS, 1977, 36 (03) : 884 - 884

← 1 2 3 4 5 →