The molecular biology of Euglena gracilis.: XV.: Recovery from centrifugation-induced stratification

被引:1
|
作者
Kempner, ES [1 ]
Miller, JH [1 ]
机构
[1] NIAMSD, NIH, Bethesda, MD 20892 USA
来源
CELL MOTILITY AND THE CYTOSKELETON | 2003年 / 56卷 / 04期
关键词
Euglena gracilis; centrifugation-induced stratification; recovery process;
D O I
10.1002/cm.10145
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The contents of Euglena gracilis cells can be separated in vivo by ultracentrifugation. Within the unbroken cell, each set of components forms a distinct layer according to their respective densities. The degree of segregation increases with both the g-force and the time of centrifugation, up to a maximum at 100,000 X g for 1 h, when six distinct strata can be observed. When returned to normal growth conditions, essentially all the cells return to the normal state and growth pattern. Greater g-forces or longer exposures do not alter the observable strata, but the ability of the cells to recover is diminished. Smaller g-forces result in less separation of cellular contents and all cells recover, even after 18 h of exposure. Euglena cells stratified at 100,000 X g for 1 h were returned to normal growth conditions; recovery was followed microscopically and by the rate of utilization of oxygen as well as that of the single carbon source. The cells recovered their normal state within 1 to 2 h, which is only a tenth of the normal doubling time. The mechanism for this recovery involves a natural process of change in cell shape caused by contraction and relaxation of the pellicle, a cell surface structure.
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页码:219 / 224
页数:6
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