Transcriptional regulation of CacyBP/SIP gene and the influence of increased CacyBP/SIP level on gene expression pattern in colorectal cancer HCT116 cells

被引：6

作者：

Kadziolka, Beata ^{[1
]}

Debski, Konrad J. ^{[1
]}

Bieganowski, Pawel ^{[2
]}

Lesniak, Wieslawa ^{[1
]}

Filipek, Anna ^{[1
]}

机构：

[1] Polish Acad Sci, Nencki Inst Expt Biol, Dept Mol & Cellular Neurobiol, Warsaw, Poland

[2] Polish Acad Sci, Dept Expt Pharmacol, Mossakowski Med Res Ctr, Warsaw, Poland

来源：

IUBMB LIFE | 2018年 / 70卷 / 01期

关键词：

CacyBP; SIP; CREB; E2F1; EGR1; gene expression; microarrays; E2F FAMILY-MEMBERS; NEUROBLASTOMA NB2A; EMERGING ROLES; PROTEIN; RESPONSES; PATHWAY; TARGET; GROWTH; CREB; PROLIFERATION;

D O I：

10.1002/iub.1698

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The CacyBP/SIP protein is expressed at a particularly high level in brain, spleen, and various tumors. In this work, we have studied transcriptional regulation of the CacyBP/SIP gene and the influence of increased CacyBP/SIP level on gene expression in colorectal cancer HCT116 cells. We have shown that E2F1, EGR1, and CREB transcription factors bind to the CacyBP/SIP gene promoter and stimulate transcription of CacyBP/SIP gene. The role of CREB was further confirmed by the observation that forskolin, a strong activator of CREB phosphorylation/activity, increased CacyBP/SIP gene promoter activity. Moreover, we have shown that CREB dominant negative mutants, CREB133 and KCREB, inhibits CacyBP/SIP promoter activity. To check the biological significance of increased CacyBP/SIP expression/level we have applied RNA microarray analysis and have found that upregulation of CacyBP/SIP entails changes in mRNA level of many genes involved, among others, in immune processes. (c) 2017 IUBMB Life, 70(1):50-59, 2018

引用

页码：50 / 59

页数：10

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