Strategy for Modular Tagged High-Throughput Amplicon Sequencing

被引：22
|
作者：
de Carcer, Daniel Aguirre [1 ]
Denman, Stuart E. [1 ]
McSweeney, Chris [1 ]
Morrison, Mark [1 ]
机构：
[1] CSIRO Preventat Hlth Natl Res Flagship & Livestoc, Queensland Biosci Precinct, St Lucia, Qld 4067, Australia
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2011年 / 77卷 / 17期
关键词：
BACTERIA; FLORA;
D O I：
10.1128/AEM.05146-11
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The use and validation of a strategy that allows a universal set of bar-coded sequencing primers to be appended to an amplified PCR product is described. The strategy allows a modular approach, in that the same bar code can be used with two or more target-specific primer sets, even simultaneously.
引用
收藏
页码：6310 / 6312
页数：3
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