Biological and molecular characterization of Tunisian isolates of Potato leafroll virus

被引:0
|
作者
Khouadja, FD
Rouzé-Jouan, J
Guyader, S
Marrakchi, M
Fakhfakh, H
机构
[1] Fac Sci Tunis, Lab Genet Mol Immunol & Biotechnol, Tunis 2092, Tunisia
[2] INRA Rheu, UMR BiO3P, INRA,Lab Zool, ENSAR Biol Organismes & Populat Appl Protect Plan, F-35653 Rennes, France
关键词
ORF0; sequence; Myzus persicae; Physalis foridana; potato; PLRV symptoms;
D O I
暂无
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Nine Tunisian isolates of Potato leafroll virus (PLRV) were collected from potato plants (cv Spunta) with similar secondary leafroll symptoms. These PLRV isolates were readily transmitted by Myzus persicae (Sulzer) from potato plants to Physalis foridana test plants inducing symptoms of differing severities. In order to investigate the genetic variability between the PLRV isolates, the ORFO regions of the Tunisian PLRV isolates were sequenced and compared with that of a French isolate (Fr5) that induces mild symptoms and 24 ORFO PLRV sequences available in Genbank. The Tunisian isolate;; could be classified in two of the three groupings of the phylogenetic tree obtained. A tentative correlation b, tween symptom expression in P. foridana and specific changes in the ORFO sequences is discussed.
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页码:91 / 99
页数:9
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