Antigenic Characterization of Expressed Complete and Different Truncated Recombinant Nucleocapsid Proteins of Hantaan Virus by Monoclonal Antibodies

被引：0
|
作者：
Lu, Xin [1 ]
Zhang, Fanglin [1 ]
Li, Yucheng [2 ]
Xue, Xiaoping [1 ]
Yin, Wen [1 ]
Xu, Zhikai [1 ]
机构：
[1] Fourth Mil Med Univ, Dept Microbiol, Coll Stomatol, Xian 710032, Shaanxi Provinc, Peoples R China
[2] Fourth Mil Med Univ, Dept Endodont, Coll Stomatol, Xian 710032, Shaanxi Provinc, Peoples R China
来源：
HYBRIDOMA | 2011年 / 30卷 / 05期
基金：
中国国家自然科学基金;
关键词：
HANTAVIRUS INFECTIONS; HEMORRHAGIC-FEVER; RENAL SYNDROME; DOMAIN; SEOUL; STRAIN; REGION; PANEL;
D O I：
10.1089/hyb.2011.0016
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The nucleocapsid protein (NP) of hantavirus has been shown to be highly immunogenic in laboratory animals and humans, and it induces an early and long-lasting humoral immune response during hantavirus infection. In the present study, entire and three partially truncated open reading frames (ORF) of NP genes of Hantaan virus (HTNV) strain 76-118, a prototype of hantavirus, were amplified and then cloned in prokaryotic expression vectors. The recombinant whole NP and truncated NPs were expressed in Escherichia coli. In addition, their antigenic and biological activities were evaluated in an enzyme-linked immunosorbent assay (ELISA) for detection of a series of monoclonal antibodies (MAbs). Results showed that the expressed complete HTNV NP had a similar function as an authentic viral NP. The antigen sites on NP of hantavirus were mainly located in the N-terminus, and the region at 1-37 amino acid might be one important antigenic domain.
引用
收藏
页码：445 / 450
页数：6
相关论文
共 50 条
  • [1] Antigenic characterization of Hantaan and Seoul virus nucleocapsid proteins expressed by recombinant baculovirus: Application of a truncated protein, lacking an antigenic region common to the two viruses, as a serotyping antigen
    Morii, M
    Yoshimatsu, K
    Arikawa, J
    Zhou, GZ
    Kariwa, H
    Takashima, I
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (09) : 2514 - 2521
  • [2] DEVELOPMENT OF MONOCLONAL-ANTIBODIES AGAINST HANTAAN VIRUS NUCLEOCAPSID PROTEIN
    HA, IS
    CHOI, Y
    PARK, YS
    CHEONG, HI
    KOO, JW
    KIM, IS
    KIM, EC
    KIM, S
    LEE, JS
    [J]. CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 1995, 2 (04) : 439 - 442
  • [3] CHARACTERIZATION OF HANTAAN VIRUS ENVELOPE GLYCOPROTEIN ANTIGENIC DETERMINANTS DEFINED BY MONOCLONAL-ANTIBODIES
    ARIKAWA, J
    SCHMALJOHN, AL
    DALRYMPLE, JM
    SCHMALJOHN, CS
    [J]. JOURNAL OF GENERAL VIROLOGY, 1989, 70 : 615 - 624
  • [4] Characterization of the nucleocapsid protein of Hantaan virus strain 76-118 using monoclonal antibodies
    Yoshimatsu, K
    Arikawa, J
    Tamura, M
    Yoshida, R
    Lundkvist, A
    Niklasson, B
    Kariwa, H
    Azuma, I
    [J]. JOURNAL OF GENERAL VIROLOGY, 1996, 77 : 695 - 704
  • [5] Characterization of Tula virus antigenic determinants defined by monoclonal antibodies raised against baculovirus-expressed nucleocapsid protein
    Lundkvist, A
    Vapalahti, O
    Plyusnin, A
    Sjolander, KB
    Niklasson, B
    Vaheri, A
    [J]. VIRUS RESEARCH, 1996, 45 (01) : 29 - 44
  • [6] Antigenic characterization of the nucleocapsid protein of Newcastle disease virus by means of a new panel of monoclonal antibodies
    Panshin, A
    Shihmanter, E
    Weisman, Y
    Örvell, C
    Kydyrmanov, A
    Asanov, N
    Daulbaeva, K
    Sayatov, M
    Lipkind, M
    [J]. COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 2000, 23 (03) : 209 - 220
  • [7] DEMONSTRATION OF ANTIGENIC VARIATION AMONG RABIES VIRUS ISOLATES BY USING MONOCLONAL-ANTIBODIES TO NUCLEOCAPSID PROTEINS
    SMITH, JS
    REIDSANDEN, FL
    ROUMILLAT, LF
    TRIMARCHI, C
    CLARK, K
    BAER, GM
    WINKLER, WG
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1986, 24 (04) : 573 - 580
  • [8] Detection of antibodies to respiratory syncytial virus attachment and nucleocapsid proteins with recombinant baculovirus-expressed antigens
    Buraphacheep, W
    Britt, WJ
    Sullender, WM
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (02) : 354 - 357
  • [9] ANTIGENIC CHARACTERIZATION OF THE INTERNAL PROTEINS OF NEWCASTLE-DISEASE VIRUS BY MONOCLONAL-ANTIBODIES
    NISHIKAWA, K
    HANADA, N
    MORISHIMA, T
    YOSHIDA, T
    HAMAGUCHI, M
    TOYODA, T
    NAGAI, Y
    [J]. VIRUS RESEARCH, 1987, 7 (01) : 83 - 92
  • [10] PROTECTIVE ROLE OF ANTIGENIC SITES ON THE ENVELOPE PROTEIN OF HANTAAN VIRUS DEFINED BY MONOCLONAL-ANTIBODIES
    ARIKAWA, J
    YAO, JS
    YOSHIMATSU, K
    TAKASHIMA, I
    HASHIMOTO, N
    [J]. ARCHIVES OF VIROLOGY, 1992, 126 (1-4) : 271 - 281
12345