Filter Papers to Collect Blood Samples from Dogs: An Easier Way to Monitor the Mass Vaccination Campaigns against Rabies?

被引：1

作者：

Wasniewski, Marine ^{[1
]}

Barrat, Jacques ^{[1
]}

Ben Maiez, Samia ^{[2
]}

Kharmachi, Habib ^{[2
]}

Handous, Mariem ^{[2
]}

Cliquet, Florence ^{[1
]}

机构：

[1] ANSES, WHO Collaborating Ctr Res & Management Zoonoses C, OIE Reference Lab Rabies,European Union Reference, Technopole Agr & Veterinaire,Nancy Lab Rabies & W, F-40009 Malzeville, France

[2] Pasteur Inst Tunis, Lab Rabies, 13 Pl Pasteur, Tunis 1002, Tunisia

来源：

VIRUSES-BASEL | 2022年 / 14卷 / 04期

关键词：

rabies virus; filter paper; dogs; ELISA; FAVN test; mass vaccination; ANTIBODIES; FOXES; ELISA; ELIMINATION; COVERAGE; IMPACT;

D O I：

10.3390/v14040711

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Rabies is a deadly viral disease present mainly in low-income countries of Africa and Asia. Dogs are the main reservoir and the source of human deaths. Mass vaccination campaigns of dogs are pivotal to achieve rabies elimination. The monitoring of the immune response of the dog population is necessary to evaluate the effectiveness of these campaigns, taking into account field conditions. This study explores the feasibility and the performance of a new tool using filter papers (FPs) to collect blood samples associated with an Enzyme-Linked ImmunoSorbent Assay (ELISA) titration of rabies antibodies in dogs. A total of 216 eluates from FP samples were collected from 111 dogs kept in experimental facilities in France and 29 dogs from the field in Tunisia. Sera were also analyzed using both the Fluorescence Antibody Virus Neutralization test (FAVNt) and ELISA. A high specificity (98.0%) was obtained by testing FP blood eluates from 51 unvaccinated dogs, with the results compared with those of FAVNt and ELISA on serum samples. The coefficients of concordance between FP eluates and tested sera were 88.9% for FAVNt and 88.0% for ELISA. Blood filter papers coupled with the titration of rabies antibodies by ELISA provide a reliable, simple, and effective solution to overcome the issues of the logistics and transport of samples, especially in low-income countries.

引用

页数：10