Oral bacterial DNA-based discrimination of human and canine saliva for the analysis of indistinct bite marks

被引:2
|
作者
Ohta, Jun [1 ,2 ]
Konishi-Kato, Yuko [3 ]
Minegishi, Saki [1 ]
Sakurada, Koichi [1 ]
机构
[1] Tokyo Med & Dent Univ, Grad Sch Med & Dent Sci, Dept Forens Dent, Bunkyo Ku, 1-5-45 Yushima, Tokyo 1138510, Japan
[2] Kanagawa Prefectural Police, Sci Crime Lab, Naka Ku, 155-1 Yamashita Cho, Yokohama, Kanagawa 2310023, Japan
[3] Hyogo Prefectural Police Headquarters, Forens Sci Lab, Chuo Ku, 5-4-1 Shimoyamatedo Ri, Kobe, Hyogo 6508510, Japan
关键词
Bite mark analysis; Human saliva; Canine saliva; Streptococcus oralis; Pasteurella canis; Duplex PCR; BODY-FLUID IDENTIFICATION; FORENSIC APPROACH; SCIENTIFIC BASIS; DOG-BITE; STREPTOCOCCI; PERSISTENCE; SKIN;
D O I
10.1016/j.fsigen.2021.102566
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Analyzing ambiguous bite marks using conventional morphological approaches to identify attackers is difficult; thus, applying molecular biological methods for identifying an attacker from their saliva is a possible approach in a forensic investigation. This study aimed to establish oral bacterial DNA-based human and canine saliva markers and develop a practical method for their discrimination. We considered Streptococcus oralis and Pasteurella canis as human and canine saliva marker candidates, respectively. Duplex bacterial DNA detection using melting curve analysis was designed and evaluated for forensic applicability using proof-of-concept experiments. S. oralis DNA was detected from human saliva samples from 30 out of 30 individuals, and P. canis DNA was detected from canine saliva samples from 73 out of 77 individuals (26 dog breeds). Additionally, both bacterial DNA markers were accurately detected from human blood-contaminated saliva samples and mock indistinct bite marks. Our results indicate that both bacterial DNA markers were sensitive, robust, and discriminating saliva markers. We consider that our duplex bacterial DNA examination is a simple, practical, and useful method for the detection of saliva from indistinct bite marks and discrimination between human and canine saliva.
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页数:8
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