Microfluidic Cell Chip Analytical System with LED Induced Fluorescence Detector

Based on micro-electro-mechanical systems technique and microfluidic cell chip analytical methodology, a microfluidic cell chip analytical system with light emitting diode(LED) induced fluorescence detector was constructed for monitoring cells in the suspensions with a low concentration below 40 Cell/mL. Cells passed through the micro-channels on the designed microchip, and the target cells labeled by 5- or 6-(N-succinimidyloxycarbonyl) -3',6'-0, 0'-diacetylfluorescein (CFDA SE) were stimulated by the LED to produce fluorescence signals which were collected by a photomultiplier tube from the roof window and changed into electrical signals finally. The HepG2 hepatoma cells labeled by CFDA SE in samples could be detected and counted by this analytical microsystem. The designed microsystem ran effectively with the coexistence of blood cells. The whole microsystem is simple, convenient and high sensitivity.