Effect of manoalide on apoptosis induced by deprivation of growth factors in vascular endothelial cells

被引：0
|
作者：
Miao, JY [1 ]
Araki, S
Zhang, HW
Hayashi, H
机构：
[1] Shandong Univ, Sch Life Sci, Inst Dev Biol, Jinan 250100, Peoples R China
[2] Nagoya Univ, Sch Sci, Sugashima Marine Biol Lab, Toba, Mie 517, Japan
来源：
ACTA PHARMACOLOGICA SINICA | 1999年 / 20卷 / 02期
关键词：
manoalide; apoptosis; umbilical veins; vascular endothelium; cultured cells; cell survival; DNA fragmentation; acidic fibroblast growth factor;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
AIM: To study effect of manoalide on apoptosis induced by deprivation of acidic fibroblast growth factor (aFGF) and serum in vascular endothelial cells ( VEC). METHODS: Morphologic changes were observed by light microscopy. Viability was determined by counting the cells that attached to dishes after treatments. DNA fragmentation was analyzed by agarose gel electrophoresis and fluorescence microscopy. RESULTS: The cells deprived of aFGF and serum were exposed to manoalide 1 - 4 mu mol.L-1 for 48 h, detachment and DNA fragmentation of these cells were suppressed. At 7 mu mol.L-1, manoalide promoted detachment and DNA fragmentation of VEC. CONCLUSION: manoalide 2 mu mol L-1 inhibited, but 7 mu mol.L-1 promoted, apoptosis of VEC.
引用
收藏
页码：121 / 125
页数：5
相关论文
共 50 条
  • [1] EfFect of manoalide on apoptosis induced by deprivation of growth factors in vascular endothelial celLs
    苗俊英
    荒木聪彦
    张红卫
    林博司
    Acta Pharmacologica Sinica, 1999, (02) : 26 - 30
  • [2] Integrin β4 mAb inhibited apoptosis induced by deprivation of growth factors in vascular endothelial cells
    Ke-wen ZHAO
    ActaPharmacologicaSinica, 2004, (06) : 31 - 35
  • [3] Integrin β4 mAb inhibited apoptosis induced by deprivation of growth factors in vascular endothelial cells
    Zhao, KW
    Zhao, QT
    Zhang, SL
    Miao, JY
    ACTA PHARMACOLOGICA SINICA, 2004, 25 (06) : 733 - 737
  • [4] 1-methyl-3-isobutylxanthine delays apoptosis induced by deprivation of growth factors in vascular endothelial cells
    Miao, JY
    Araki, S
    Zhang, SL
    Hayashi, H
    ACTA PHARMACOLOGICA SINICA, 2000, 21 (10): : 936 - 938
  • [5] Effect of safrole oxide on vascular endothelial cell growth and apoptosis induced by deprivation of fibroblast growth factor
    Miao, JY
    Zhao, BX
    Li, HH
    Zhang, SL
    Du, CQ
    ACTA PHARMACOLOGICA SINICA, 2002, 23 (04) : 323 - 326
  • [6] APOPTOSIS OF VASCULAR ENDOTHELIAL-CELLS BY FIBROBLAST GROWTH-FACTOR DEPRIVATION
    ARAKI, S
    SHIMADA, Y
    KAJI, K
    HAYASHI, H
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 168 (03) : 1194 - 1200
  • [7] Glycine prevents apoptosis of rat sinusoidal endothelial cells caused by deprivation of vascular endothelial growth factor
    Zhang, YJ
    Ikejima, K
    Honda, H
    Kitamura, T
    Takei, Y
    Sato, N
    HEPATOLOGY, 2000, 32 (03) : 542 - 546
  • [8] Both senescence and apoptosis induced by deprivation of growth factors were inhibited by a novel butyrolactone derivative through depressing integrin β4 in vascular endothelial cells
    Wang, Weiwei
    Liu, Xia
    Zhang, Yun
    Zhao, Jing
    Zhao, Baoxiang
    Zhang, Shangli
    Miao, Junying
    ENDOTHELIUM-JOURNAL OF ENDOTHELIAL CELL RESEARCH, 2007, 14 (06): : 325 - 332
  • [9] Glycine prevents apoptosis of sinusoidal endothelial cells due to vascular endothelial growth factor (VEGF) deprivation.
    Zhang, YJ
    Ikejima, K
    Takei, Y
    Honda, H
    Hirose, M
    Fukuda, T
    Kitamura, T
    Oide, H
    Miyazaki, A
    Watanabe, S
    Sato, N
    HEPATOLOGY, 1999, 30 (04) : 225A - 225A
  • [10] Effect of vascular endothelial growth factor and epidermal growth factor on iatrogenic apoptosis in human endothelial cells
    Vinci, MC
    Visentin, B
    Cusinato, F
    Nardelli, GB
    Trevisi, L
    Luciani, S
    BIOCHEMICAL PHARMACOLOGY, 2004, 67 (02) : 277 - 284
12345