HSP90/70 chaperones are required for rapid nucleosome removal upon induction of the GAL genes of yeast

被引：56
|
作者：
Floer, Monique [1 ]
Bryant, Gene O. [1 ]
Ptashne, Mark [1 ]
机构：
[1] Sloan Kettering Inst, Program Mol Biol, New York, NY 10021 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2008年 / 105卷 / 08期
关键词：
transcription; Gal4; activation; recruitment; promoter;
D O I：
10.1073/pnas.0800053105
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Induction of transcription of the GAL genes of yeast by galactose is a multistep process: Galactose frees the activator Gal4 of its inhibitor, Gal80, allowing Gal4 to recruit proteins required to transcribe the GAL genes. Here, we show that deletion of components of either the HSP90 or the HSP70 chaperone machinery delays this induction. This delay remains when the galactose-signaling pathway is bypassed, and it cannot be explained by a chaperone requirement foe DNA binding by Gal4. Removal of promoter-bound nucleosomes is delayed in a chaperone mutant, and our findings suggest an involvement of HSP90 and HSP70 in this early step in gene induction.
引用
收藏
页码：2975 / 2980
页数：6
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