Magnetic resonance measurements of cellular and sub-cellular membrane structures in live and fixed neural tissue

被引：39

作者：

Williamson, Nathan H. ^{[1
]}

Ravin, Rea ^{[1
,2
]}

Benjamini, Dan ^{[1
,3
]}

Merkle, Hellmut ^{[4
]}

Falgairolle, Melanie ^{[4
]}

O'Donovan, Michael James ^{[4
]}

Blivis, Dvir ^{[4
]}

Ide, Dave ^{[4
,5
]}

Cai, Teddy X. ^{[1
]}

Ghorashi, Nima S. ^{[6
]}

Bai, Ruiliang ^{[1
,7
]}

Basser, Peter J. ^{[1
]}

机构：

[1] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, NIH, Bethesda, MD 20814 USA

[2] Celoptics, Rockville, MD USA

[3] Henry Jackson Fdn, Ctr Neurosci & Regenerat Med, Bethesda, MD USA

[4] NINDS, NIH, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA

[5] NIMH, NIH, Bethesda, MD 20892 USA

[6] NHLBI, Cardiovasc Branch, NIH, Bldg 10, Bethesda, MD 20892 USA

[7] Zhejiang Univ, Interdisciplinary Inst Neurosci & Technol, Sch Med, Hangzhou, Peoples R China

来源：

ELIFE | 2019年 / 8卷

关键词：

AXON DIAMETER DISTRIBUTION; GRADIENT WAVE-FORMS; SPIN-ECHO ANALYSIS; WATER DIFFUSION; RESTRICTED DIFFUSION; SELF-DIFFUSION; MICROSCOPIC ANISOTROPY; INTRACELLULAR WATER; HUMAN ERYTHROCYTES; HIPPOCAMPAL SLICE;

D O I：

10.7554/eLife.51101

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We develop magnetic resonance (MR) methods for real-time measurement of tissue microstructure and membrane permeability of live and fixed excised neonatal mouse spinal cords. Diffusion and exchange MR measurements are performed using the strong static gradient produced by a single-sided permanent magnet. Using tissue delipidation methods, we show that water diffusion is restricted solely by lipid membranes. Most of the diffusion signal can be assigned to water in tissue which is far from membranes. The remaining 25% can be assigned to water restricted on length scales of roughly a micron or less, near or within membrane structures at the cellular, organelle, and vesicle levels. Diffusion exchange spectroscopy measures water exchanging between membrane structures and free environments at 100 s(-1).

引用

页数：39

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