Multiplex RT-PCR with broad-range primers and an exogenous internal amplification control for the detection of honeybee viruses in bumblebees

被引：28
|
作者：
Meeus, Ivan [1 ,2 ]
Smagghe, Guy [1 ]
Siede, Reinhold [3 ]
Jans, Kris [4 ]
de Graaf, Dirk C. [2 ]
机构：
[1] Univ Ghent, Dept Crop Protect, Lab Agrozool, Fac Biosci Engn, B-9000 Ghent, Belgium
[2] Univ Ghent, Fac Sci, Dept Physiol, Lab Zoophysiol, B-9000 Ghent, Belgium
[3] Bieneninst Kirchhain, D-35274 Kirchhain, Germany
[4] Biobest NV, B-2260 Westerlo, Belgium
来源：
JOURNAL OF INVERTEBRATE PATHOLOGY | 2010年 / 105卷 / 02期
关键词：
Acute bee paralysis virus; Kashmir bee virus; Deformed wing virus; Multiplex RT-PCR; RNA-dependent RNA polymerase; Bumblebees; APIS-MELLIFERA L; ACUTE PARALYSIS VIRUS; DEFORMED-WING-VIRUS; ACUTE-BEE-PARALYSIS; VARROA-DESTRUCTOR; GENETIC-ANALYSIS; 1ST DETECTION; POPULATIONS; COMMERCIALIZATION; INFECTION;
D O I：
10.1016/j.jip.2010.06.012
中图分类号：
Q95 [动物学];
学科分类号：
071002 ;
摘要：
Bumblebees are commercially reared and transported worldwide mainly for pollination of greenhouse tomatoes. Three honeybee viruses have been reported in bumblebees: Acute bee paralysis virus, Kashmir bee virus and Deformed wing virus. We developed a multiplex RT-PCR with primers designed on highly conserved regions of the RNA-dependent RNA polymerase in order to detect a maximum range of viral variants. Rearing facilities and governmental organizations can now thoroughly screen bumblebee colonies with a cost-effective technique with an integrated internal amplification control (IAC) implementable in laboratories that strive for International Organization for Standardization (ISO) certification. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码：200 / 203
页数：4
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