Isolation and characterization of microsatellite markers for Cedrela odorata L. (Meliaceae), a high value neotropical tree

被引:14
|
作者
Hernandez, G. [1 ,2 ]
Buonamici, A. [3 ]
Walker, K. [1 ]
Vendramin, G. G. [3 ]
Navarro, C. [2 ]
Cavers, S. [1 ]
机构
[1] CEH Edinburgh, NERC Ctr Ecol & Hydrol, Penicuik EH26 0QB, Midlothian, Scotland
[2] Ctr Agronom Trop Invest & Ensenanza, Turrialba, Costa Rica
[3] CNR, Plant Genet Inst, I-50019 Florence, Italy
基金
英国自然环境研究理事会;
关键词
Cedrela odorata; cedar; meliaceae; microsatellites;
D O I
10.1007/s10592-007-9334-y
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
We describe 9 primers for amplification of microsatellite loci for the Neotropical tree Cedrela odorata L. (Meliaceae). Loci were isolated from an enriched library derived from a single DNA sample from a tree in Costa Rica. Levels of polymorphism were determined using samples from a large progeny trial. Across loci, the number of alleles ranged from 14 to 30. Observed heterozygosity levels ranged from 0.61 to 0.88. No linkage disequilibria were detected although some departures from Hardy-Weinberg equilibrium (HWE) were found, probably due to a Wahlund effect.
引用
收藏
页码:457 / 459
页数:3
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