TaqMan real-time PCR for detection and quantitation of squash leaf curl virus in cucurbits

被引：8
|
作者：
Kuan, Cheng-Ping [1 ]
Huang, Hung-Chang [1 ]
Chang, Chia-Che [2 ]
Lu, Yi-Lin [1 ]
机构：
[1] Taiwan Agr Res Inst, Div Biotechnol, Taichung 41362, Taiwan
[2] Natl Chung Hsing Univ, Inst Biomed Sci, Taichung 40227, Taiwan
来源：
JOURNAL OF VIROLOGICAL METHODS | 2012年 / 179卷 / 02期
关键词：
Squash leaf curl virus; Squash; Cucurbita pepo; Melon; Cucumis melo; Real-time PCR; COMPLETE NUCLEOTIDE-SEQUENCE; MOLECULAR CHARACTERIZATION; GEMINIVIRUS; BEGOMOVIRUS; SEROLOGY; VECTOR;
D O I：
10.1016/j.jviromet.2011.11.024
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A real-time PCR assay based on the TaqMan chemistry was developed for reliable detection and quantitation of the squash leaf curl virus (SLCV) in melon and squash plants. This method was highly specific to SLCV and it was about one thousand times more sensitive than the conventional PCR method. The protocol of the real-time PCR established in this study enabled detection of as little as 10(2) copies of SLCV DNA with CP gene as the target. This TaqMan real-time PCR assay for detection and quantitation of SLCV would be a useful tool for application in quarantine and certification of SLCV in cucurbits as well as in the research of disease resistance and epidemiology. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：367 / 372
页数：6
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