Evaluation of joint effects of perfluorooctane sulfonate and wood vinegar on planarians, Dugesia japonica

Perfluorooctane sulfonate (PFOS) is a persistent organic pollutant and can cause oxidative stress in animals. Wood vinegar (WV) is the water condensate of smoke produced during wood carbonization. It was used for antibacterial application, pest control, and antioxidant. In the study, PFOS and WV were used to treat the planarian, and then the oxidative stress induced by PFOS on the planarian (Dugesia japonica) and the protective effects of WV on lipid peroxidation, related antioxidant enzyme activity, and mRNA expression in the planarian were studied. PFOS caused an increase in malondialdehyde (MDA) contents, a decrease in superoxide dismutase (SOD) and catalase (CAT) activities, and a change in glutathione peroxidase (GPx), glutathione S-transferase (GST), glutathione reductase (GR) activities. The mRNA levels of glutathione peroxidase gene (gpx), glutathione S-transferase enzyme gene (gst), and glutathione reductase gene (gr) are upregulated or downregulated to varying degrees. The WV and co-treatment planarians reduced MDA levels, increased the activities of oxidative stress biomarker enzymes, and restored gene expression levels. Our results show that low concentration of WV has protective effects on the oxidative damage caused by PFOS in the planarian.