Pilot-scale production of carboxymethylcellulase from rice hull by Bacillus amyloliquefaciens DL-3

被引：35

作者：

Jo, Kang-Ik ^{[1
]}

Lee, You-Jung ^{[1
]}

Kim, Bo-Kyung ^{[1
]}

Lee, Bo-Hwa ^{[1
]}

Chung, Chung-Han ^{[1
]}

Nam, Soo-Wan ^{[2
]}

Kim, Sung-Koo ^{[3
]}

Lee, Jin-Woo ^{[1
]}

机构：

[1] Dong A Univ, Coll Nat Res & Life Sci, Dept Biotechnol, Pusan 604714, South Korea

[2] Dong Eui Univ, Coll Engn, Dept Biotechnol & Bioengn, Pusan 614714, South Korea

[3] Pukyong Natl Univ, Coll Fisheries Sci, Dept Biotechnol & Bioengn, Pusan 608737, South Korea

来源：

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2008年 / 13卷 / 02期

关键词：

Bacillus amyloliquefaciens; carboxymethylcellulase (CMCase); optimization; rice hull;

D O I：

10.1007/s12257-007-0149-y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Optimal conditions for pilot-scale production of the carboxymethylcellulase (CMCase) by Bacillus amyloliquefaciens DL-3 were investigated. The best carbon and nitrogen sources for the production of CMCase by B. amyloliquefaciens DL-3 were found to be rice hull and peptone and their optimal concentrations were 5.0 and 0.20% (w/v), respectively. Optimal temperature and initial pH for the production of CMCase were 37 C and 6.8. Optimal agitation speed and aeration rate for the production of CMCase were 300 rpm and 1.0 vvm in a 7 L bioreactor, which were different from those for the cell growth of B. amyloliquefaciens DL-3. The highest productions of CMCase by B. amyloliquefaciens DL-3 from 5.0% (w/v) rice hull as a carbon source under optimal conditions in a 7 or 100 L bioreactor were 220 and 367 U/mL, respectively. (C) KSBB.

引用

页码：182 / 188

页数：7

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