Electrochemical and analytical applications for NADH detection at glassy carbon electrode modified with nickel nanoparticles dispersed on poly 1,5-diaminonaphthalene

An electrochemical sensor based on glassy carbon electrode (GC) modified with nickel nanoparticles (NiNPs) dispersed on poly 1,5-diaminonaphthalene (PDAN) to perform nickel nanoparticles/poly 1,5-diaminonaphthalene/GC (NiNPs/PDAN/GC)-modified electrode (ME) was used for the determination of dihydronicotinamide adenine dinucleotide (NADH) using cyclic voltammetry (CV), square wave voltammetry (SWV), and differential pulse voltammetry (DPV) techniques in basic medium. Electrochemical studies showed that NiNPs/PDAN/GC ME provides a positively synergistic effect on the electrochemical oxidation of NADH. The electrocatalytic currents obtained for the three techniques were linearly related to NADH concentrations. The obtained linear concentration calibration plots gave low detection limits (LOD) of 0.378, 0.122, and 0.02 mu M for CV, SWV, and DPV techniques, respectively, while the low quantification limits (LOQ) were 1.259, 0.408, and 0.067 mu M, respectively. Linear detection ranges (LDR) calculated were 300-7000, 100-1000, and 10-150 mu M for the three techniques, respectively. Interference studies showed that the ME exhibits excellent selectivity toward NADH.