Molecular typing of Brucella species isolates from livestock and human

被引：23
|
作者：
Nagalingam, Mohandoss [1 ]
Shome, Rajeswari [1 ]
Balamurugan, Vinayagamurthy [1 ]
Shome, Bibek Ranjan [1 ]
NarayanaRao, Krishnamsetty [1 ]
Vivekananda [2 ]
Isloor, Shrikrishna [2 ]
Prabhudas, Krishnamsetty [1 ]
机构：
[1] Project Directorate Anim Dis Monitoring & Surveil, Bangalore 560024, Karnataka, India
[2] KAVFSU, Dept Microbiol, Vet Coll, Bangalore 560024, Karnataka, India
来源：
TROPICAL ANIMAL HEALTH AND PRODUCTION | 2012年 / 44卷 / 01期
关键词：
AMOS PCR; Brucella; Indian isolates; Typing-multiplex PCR; MULTIPLEX PCR ASSAY; VACCINE STRAINS; DIFFERENTIATION; MELITENSIS; RB51; S19;
D O I：
10.1007/s11250-011-9886-1
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Although host specificity has been observed in different species of Brucella, crossing the animal host boundary is likely to occur at any time. In this study, Bruce ladder PCR and abortus-melitensis-ovis-suis (AMOS) PCR assays were used to characterize 47 Brucella isolates from Indian origin in order to know exact species for understanding epidemiology of brucellosis. Out of them, 28, 14, and 5 isolates were found to be Brucella abortus, Brucella melitensis, and Brucella suis, respectively. Further analysis by AMOS PCR has identified that all the B. abortus isolates belong to any one of the biovar 1, 2, or 4; of the five B. suis isolates, three belong to biovar 1 and two belong to any one of the biovar 2, 3, 4, or 5. Although this multiplex Bruce ladder PCR is useful in differentiating all Brucella species, elaborate study is required to further characterize the isolates at exact biovar level.
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页码：5 / 9
页数：5
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