Whole-mount in situ hybridization to mouse embryos

被引：44
|
作者：
Correia, KM [1 ]
Conlon, RA [1 ]
机构：
[1] Case Western Reserve Univ, Univ Hosp, Dept Genet, Cleveland, OH 44106 USA
来源：
METHODS | 2001年 / 23卷 / 04期
关键词：
D O I：
10.1006/meth.2000.1145
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The mouse is well-established as the major animal model for the study of mammalian development. Rapid progress in large-scale cDNA and also genomic sequencing projects is identifying new mouse genes at an unprecedented rate. As a first step toward understanding the function of these novel genes, it is important to determine their developmental expression pattern. Here we provide a reliable, sensitive method for wholemount in situ hybridization using the mouse embryo. (C) 2001 Academic Press.
引用
收藏
页码：335 / 338
页数：4
相关论文
共 50 条
  • [1] Fluorescence in situ hybridization in whole-mount Drosophila embryos
    Hughes, SC
    SaulierLeDrean, B
    LivneBar, I
    Krause, HM
    BIOTECHNIQUES, 1996, 20 (05) : 748 - 750
  • [2] Multicolour whole-mount in situ hybridization to Drosophila embryos
    Hauptmann, G
    Gerster, T
    DEVELOPMENT GENES AND EVOLUTION, 1996, 206 (04) : 292 - 295
  • [3] An optimized procedure for whole-mount in situ hybridization on mouse embryos and embryoid bodies
    Piette, Denis
    Hendrickx, Marijke
    Willems, Erik
    Kemp, Caroline R.
    Leyns, Luc
    NATURE PROTOCOLS, 2008, 3 (07) : 1194 - 1201
  • [4] An economical and practical method for whole-mount in situ hybridization for mouse embryos and organs
    Pu, D.
    Du, J. L.
    Zhang, J.
    Li, X. Q.
    Weng, M. J.
    Liu, Y. J.
    Gao, L. Z.
    Xia, S.
    Chen, Y. Q.
    She, Q.
    BIOTECHNIC & HISTOCHEMISTRY, 2013, 88 (01) : 27 - 37
  • [5] An optimized procedure for whole-mount in situ hybridization on mouse embryos and embryoid bodies
    Denis Piette
    Marijke Hendrickx
    Erik Willems
    Caroline R Kemp
    Luc Leyns
    Nature Protocols, 2008, 3 : 1194 - 1201
  • [6] Double labeling for whole-mount in situ hybridization in mouse
    Hecksher-Sorensen, J
    Hill, RE
    Lettice, L
    BIOTECHNIQUES, 1998, 24 (06) : 914 - +
  • [7] Combined whole-mount in situ hybridization and immunohistochemistry in avian embryos
    Streit, A
    Stern, CD
    METHODS, 2001, 23 (04) : 339 - 344
  • [8] Postimplantation mouse embryos cultured in vitro. Assessment with whole-mount immunostaining and in situ hybridization
    vanMaeleFabry, G
    Clotman, F
    Gofflot, F
    Bosschaert, J
    Picard, JJ
    INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, 1997, 41 (02): : 365 - 374
  • [9] High-resolution in situ hybridization to whole-mount zebrafish embryos
    Christine Thisse
    Bernard Thisse
    Nature Protocols, 2008, 3 : 59 - 69
  • [10] Double labeling with fluorescence in situ hybridization in Drosophila whole-mount embryos
    Hughes, SC
    Krause, HM
    BIOTECHNIQUES, 1998, 24 (04) : 530 - 532
12345