Antimicrobial activity of the extracts from fruits of Rumex L. species

This study was designed primarily to investigate the antibacterial and antifungal activity of the extracts from fruits of six Rumex L. species: R. acetosa L., R. acetosella L., R. confertus Willd., R. crispus L., R. hydrolapathum Huds. and R. obtusifolius L. The 7 Grampositive and 7 Gram-negative bacteria strains and 5 fungal ones were tested by agar and broth dilution method. Determination of minimal inhibitory concentration (MIC) revealed that the extracts from R. confertus, R. crispus, R. hydrolapathum and R. obtusifolius exerted differential inhibitory effect on the growth of Gram-positive bacteria - staphylococci (MIC=62.5-125 A mu g/mL) and Gramnegative bacteria - Escherichia coli ATCC 3521, Proteus mirabilis, Pseudomonas aeruginosa (MIC=125 -> 500 A mu g/mL); MIC values determined by agar dilution method were somewhat higher. The same extracts inhibited also the growth of fungi - Candida spp. or Trichophyton mentagrophytes ATCC 9533 (MIC=250-500 A mu g/mL), as found by agar dilution method. The total content of polyphenols (11.66-78.36 mg/g), anthracene derivatives (0.26-12.93 mg/g) and tannins (4.00-11.16%) was also determined.