A RAPID ON-CHIP SINGLE NUCLEOTIDE POLYMORPHISM DETECTION DIRECTLY FROM HUMAN BLOOD

被引：0

作者：

Zhang, L. ^{[1
]}

Cai, Q. ^{[1
]}

Jones, B. ^{[1
]}

Fiorini, P. ^{[1
]}

Stakenborg, T. ^{[1
]}

Masahiko, T. ^{[2
]}

Lagae, L. ^{[1
,3
]}

机构：

[1] IMEC, Leuven, Belgium

[2] Panasonic Corp, Kyoto, Japan

[3] KULeuven, Dept Phys & Astron, Leuven, Belgium

来源：

2015 TRANSDUCERS - 2015 18TH INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS (TRANSDUCERS) | 2015年

关键词：

Microfluidic; lab on a chip; polymerase chain reaction; single nucleotide polymorphism; genotyping;

D O I：

暂无

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

This paper reports on a silicon/glass based microsystem for the fast detection of single nucleotide polymorphisms (SNPs) directly from on-chip filtered human blood. A cross-flow micro-pillar filter is used for purifying lysed blood. It provides an easy solution for on-chip blood sample preparation. After purification, polymerase chain reaction (PCR) was no longer inhibited. The purified DNA solution is then tested for the detection of specific SNPs in a thermally insulated micro-cavity by real-time PCR. As a proof of principle, a successful on-chip detection of two SNPs in human CYP2C9 gene is demonstrated. The SNP profiling using our device took less than 40 minutes from sample to result.

引用

页码：1669 / 1671

页数：3

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