Systematic gene microarray analysis of the lncRNA expression profiles in human uterine cervix carcinoma

被引:47
|
作者
Chen, Jie [1 ]
Fu, Ziyi [2 ]
Ji, Chenbo [2 ]
Gu, Pingqing [3 ]
Xu, Pengfei [2 ]
Yu, Ningzhu [1 ]
Kan, Yansheng [1 ]
Wu, Xiaowei [4 ]
Shen, Rong [1 ]
Shen, Yan [1 ]
机构
[1] Nanjing Med Univ, Cerv Branch, Affiliated Nanjing Maternal & Child Hlth Hosp, Nanjing 210004, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Nanjing Maternal & Child Hlth Med Inst, Affiliated Nanjing Maternal & Child Hlth Hosp, Nanjing 210004, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Dept Clin Lab, Affiliated Nanjing Maternal & Child Hlth Hosp, Nanjing 210004, Jiangsu, Peoples R China
[4] Nanjing Med Univ, Sch Basic Med Sci, Dept Pharmacol, Nanjing 210029, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
Human uterine cervix carcinoma; Microarray analysis; lncRNA; LONG NONCODING RNAS; DNA METHYLATION; PROLIFERATION; INSIGHTS; PROMOTES; HOTAIR; P53;
D O I
10.1016/j.biopha.2015.04.010
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
The human uterine cervix carcinoma is one of the most well-known malignancy reproductive system cancers, which threatens women health globally. However, the mechanisms of the oncogenesis and development process of cervix carcinoma are not yet fully understood. Long non-coding RNAs (lncRNAs) have been proved to play key roles in various biological processes, especially development of cancer. The function and mechanism of lncRNAs on cervix carcinoma is still rarely reported. We selected 3 cervix cancer and normal cervix tissues separately, then performed lncRNA microarray to detect the differentially expressed lncRNAs. Subsequently, we explored the potential function of these dysregulated lncRNAs through online bioinformatics databases. Finally, quantity real-time PCR was carried out to confirm the expression levels of these dysregulated lncRNAs in cervix cancer and normal tissues. We uncovered the profiles of differentially expressed lncRNAs between normal and cervix carcinoma tissues by using the microarray techniques, and found 1622 upregulated and 3026 downregulated lncRNAs (fold-change > 2.0) in cervix carcinoma compared to the normal cervical tissue. Furthermore, we found HOXA11-AS might participate in cervix carcinogenesis by regulating HOXA11, which is involved in regulating biological processes of cervix cancer. This study afforded expression profiles of lncRNAs between cervix carcinoma tissue and normal cervical tissue, which could provide database for further research about the function and mechanism of key-lncRNAs in cervix carcinoma, and might be helpful to explore potential diagnosis factors and therapeutic targets for cervix carcinoma. (C) 2015 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:83 / 90
页数:8
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