Determination of Proteomic and Metabolic Composition of Exhaled Breath Condensate of Newborns

被引：1

作者：

Kononikhin, A. S. ^{[1
,2
]}

Chagovets, V. V. ^{[1
]}

Starodubtseva, N. L. ^{[1
,2
]}

Ryndin, A. Y. ^{[1
]}

Bugrova, A. E. ^{[1
,3
]}

Kostyukevich, Y. I. ^{[2
,3
]}

Popov, I. A. ^{[1
,2
,3
]}

Frankevich, V. E. ^{[1
]}

Ionov, O. V. ^{[1
]}

Sukhikh, G. T. ^{[1
]}

Nikolaev, E. N. ^{[2
,3
]}

机构：

[1] Kulakov Res Ctr Obstet Gynecol & Perinatol, Moscow 117997, Russia

[2] Moscow Inst Phys & Technol, Dolgoprudnyi 141700, Moscow Oblast, Russia

[3] Emanuel Inst Biochem Phys, Moscow 119934, Russia

来源：

MOLECULAR BIOLOGY | 2016年 / 50卷 / 03期

关键词：

proteomic analysis; metabolomics; mass spectrometry; exhaled breath condensate (EBC); newborns; VOLATILE ORGANIC-COMPOUNDS; BIOMARKERS;

D O I：

10.1134/S0026893316020096

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here, the possibility of proteomic and metabolomic analysis of the composition of exhaled breath condensate of neonates with respiratory support. The developed method allows non-invasive collecting sufficient amount of the material for identification of disease-specific biomarkers. Samples were collected by using a condensing device that was incorporated into the ventilation system. The collected condensate was analyzed by liquid chromatography coupled with high resolution mass spectrometry and tandem mass spectrometry. The isolated substances were identified with a use of databases for proteins and metabolites. As a result, a number of compounds that compose the exhaled breath condensate was determined and can be considered as possible biomarkers of newborn diseases or stage of development.

引用

页码：470 / 473

页数：4

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