Intensification of large-scale cell culture processes

Manufacturers of recombinant proteins frequently face the challenge to satisfy the market needs with existing large scale production capacity. For fed-batch processes, the maximal capacity is given in yield per batch multiplied by the number of runs that can be done per year, thus strongly depending on the duration of the fed-batch run. This short review is focused on starting the fed-batch at high seeding densities, reducing the duration of the fed-batch as an efficient strategy to increase the manufacturing capacity without modifying the production bioreactor. This strategy makes fed-batch processes highly productive from the first day on.