VALIDATION OF TLC DENSITOMETRIC METHOD FOR SIMULTANEOUS ANALYSIS OF L-LEUCINE AND L-VALINE IN ADDITIVES

被引：0
|
作者：
Ivanov, Kalin [1 ]
Tsvetkova, Dobrina [2 ]
Obreshkova, Danka [2 ]
机构：
[1] Med Univ Plovdiv, Fac Pharm, Plovdiv 4000, Bulgaria
[2] Med Univ Sofia, Dept Pharmaceut Chem, Fac Pharm, Sofia 1000, Bulgaria
来源：
COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES | 2011年 / 64卷 / 10期
关键词：
L-Leucine; L-Valine; TLC; densitometry; accuracy; linearity; CHAIN AMINO-ACIDS; CHROMATOGRAPHY-MASS SPECTROMETRY; PLASMA;
D O I：
暂无
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
TLC densitometric method for simultaneous determination of L-Leucine and L-Valine was validated in respect to analytical parameters: selectivity, accuracy, precision, linearity. Accuracy was represented by the values of the degree of recovery (R) over bar (%) +/- SD: L-Leucine: 99.29 +/- 2.6 (L-200); 99.46 +/- 4.11 (L-250); 100.08 +/- 0.81 (L-300); L-Valine. 101.51 +/- 1,86 (V-80); 98.02 +/- 1.29 (V-95); 98.89 +/- 6.42 (V-150). Precision was estimated by the uncertainty of the result, determined by SD, RSD and (X) over bar divided by t.S (X) over bar: all data suit respective confidence interval. Regression curves and equations y = a.x + b show the proportional accordance between the area of spots and concentration C, with coefficient of regression (R-2) higher than 0.997.
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页码：1429 / 1434
页数：6
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